PXD030396

PXD030396 is an original dataset announced via ProteomeXchange.

Title	Pterosin B treatment protect cells from glutamate-induced cytotoxicity
Description	The prevalence of neurodegenerative diseases is rapidly increasing with the aging trend of global populations, severely threatening human health and burdening society. However, to date, there is no effective treatment able to halt the progression or cure the diseases. Here, We aim to develop effective treatments for neurodegenerative diseases from novel sources by evaluating their neuroprotective activity and investigating the working mechanism. Primary screening on glutamate-induced excitotoxicity was assessed by MTT assay. The combination of transcriptomics and proteomics analysis were used to identify the targets of pterosin B. The key pathways were enriched by the Kyoto Encyclopedia of Genes and Genomes (KEGG). The core targets were analyzed by protein-protein interactions network by STRING. The mRNA expressions and protein expressions were evaluated using qPCR and western blot, respectively.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:00:37.802.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jordy Evan Sulaiman
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-14 08:32:12	ID requested
1	2023-02-28 20:30:33	announced
⏵ 2	2023-11-14 08:00:39	announced	2023-11-14: Updated project metadata.

Cheng A, Zhang Y, Sun J, Huang D, Sulaiman JE, Huang X, Wu L, Ye W, Wu C, Lam H, Shi Y, Qian PY, Pterosin sesquiterpenoids from Pteris laeta Wall. ex Ettingsh. protect cells from glutamate excitotoxicity by modulating mitochondrial signals. J Ethnopharmacol, 308():116308(2023) [pubmed]

submitter keyword: ROS,neuroprotective natural products, glutamate excitotoxicity, mitochondria membrane potential

Henry Lam
contact affiliation	Department of Chemical and Biological Engineering, The Hong Kong University of Science and Technology (HKUST)
contact email	kehlam@ust.hk
lab head
Jordy Evan Sulaiman
contact affiliation	The Hong Kong University of Science and Technology (HKUST)
contact email	jesulaiman@connect.ust.hk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD030396

PRIDE project URI

• PRIDE
  1. PXD030396
     1. Label: PRIDE project
     2. Name: Pterosin B treatment protect cells from glutamate-induced cytotoxicity