PXD030386

PXD030386 is an original dataset announced via ProteomeXchange.

Title	Nogo-A modulates gene expression to fine-tune enamel development
Description	Nogo-A is a major player in neural development and regeneration, and it is the target of several clinical trials. However, its functions outside the nervous system are mostly unknown. We observed that Nogo-A is expressed in dental epithelial cells, responsible for the formation of enamel, and we showed that the deletion of Nogo-A in transgenic mouse models leads to the formation of defective enamel. We observed that Nogo-A directly interacts with molecules important for gene expression regulation, and its deletion perturbs their cellular localization. As a result, Nogo-A deletion induces overexpression of genes involved in cell differentiation and enamel production. Mechanistically, we demonstrated that intracellular Nogo-A, and not cell surface Nogo-A, is responsible for gene expression modulation. Taken together, our results indicate a new role for Nogo-A as regulator of enamel formation and suggest a new possible cell-autonomous function in regulating gene expression and cell differentiation.
HostingRepository	PRIDE
AnnounceDate	2024-06-20
AnnouncementXML	Submission_2024-06-19_23:56:46.586.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Witold Wolski
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-14 06:58:45	ID requested
⏵ 1	2024-06-19 23:56:46	announced

Dataset with its publication pending

submitter keyword: Differentiation, Amelogenesis, LC-MS, Tooth, Interactome,Nogo-A, Development, Enamel, Transcriptome, DDA

Pierfrancesco Pagella
contact affiliation	Institute of Oral Biology Center of Dental Medicine (ZZM) University of Zurich (UZH)
contact email	pierfrancesco.pagella@uzh.ch
lab head
Witold Wolski
contact affiliation	Functional Genomics Center Zurch
contact email	wew@fgcz.ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD030386

PRIDE project URI

• PRIDE
  1. PXD030386
     1. Label: PRIDE project
     2. Name: Nogo-A modulates gene expression to fine-tune enamel development