PXD030384

PXD030384 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Heterologous Expression of MBP-AtABC1K6-His in E. coli
Description	Heterologous E. coli expression of AtABC1K6 tagged with maltose binding protein (MBP) and 6xHis tags revealed a spontaneous cleavage event of the recombinant protein during purification. Resulting bands were resolved on SDS-PAGE and excised for MS/MS identification. Mass spec-based identification of the individual bands determined the cleavage event as ocurring after Lysine-443. The results demonstrate that the resulting ca. 80 kD band, showing kinase activity, comprises the AtABC1K6-6xHis fragment lacking the first 71 residues of AtABC1K6.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:52:41.888.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peter Lundquist
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-14 01:38:28	ID requested
1	2022-03-02 11:58:55	announced
⏵ 2	2023-11-14 08:52:42	announced	2023-11-14: Updated project metadata.

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: COQ8,kinase, spontaneous cleavage, UbiB, ABC1

submitter keyword: COQ8,kinase, spontaneous cleavage, UbiB, ABC1

Contact List

Peter K Lundquist
contact affiliation	Department of Biochemistry & Molecular Biology, Michigan State Univeristy
contact email	pklundqu@msu.edu
lab head
Peter Lundquist
contact affiliation	Michigan State University
contact email	PKLUNDQU@MSU.EDU
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD030384
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD030384

PRIDE project URI

Repository Record List

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