PXD030372 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Sod1 Integrates Oxygen Availability to Redox Regulate NADPH Production and the Thiol Redoxome |
Description | Redox proteomics identifies additional putative targets of Sod1 redox regulation. The high abundance and broad cellular distribution of Sod1 suggests that it may function as a redox regulator of a broad variety of substrates in addition to GAPDH. To test this, we conducted a high-powered quantitative redox proteomics screen of WT and sod1Δ yeast to identify Sod1-dependent redox substrates. We used a combined SILAC-TMT approach, whereby sod1Δ-dependent changes in protein abundance are quantified through Stable Isotope Labeling with Amino acids in Cell culture (SILAC) and changes in reversible cysteine oxidation are quantified through cysteine-reactive Iodoacetyl Tandem Mass Tags (iodo-TMT) before and after reduction with DTT. Consequently, the study reveals a broad range of proteins that undergo significant changes in abundance, cysteine oxidation, or both. Focusing first on the effects of SOD1 deletion on proteome-wide protein abundance, we independently analyzed the SILAC MS data. A total of 4,409 proteins were confidently detected and quantified, and ~9% of these (373) exhibited significant differences in abundance between the two strains. Of these, 114 (30.6%) exhibit a significant decrease in protein abundance in sod1Δ cells compared to 259 (69.4%) proteins that exhibit a significant increase in abundance. Overall, we revealed a larger network of cysteine-containing proteins that are oxidized in a Sod1- dependent manner using mass spectrometry-based redox proteomics approaches. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-07 |
AnnouncementXML | Submission_2024-10-07_13:44:15.161.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Matthew Torres |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; 6x(13)C; acetylated residue; monohydroxylated residue |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-12-13 10:09:20 | ID requested | |
⏵ 1 | 2024-10-07 13:44:16 | announced | |
Publication List
10.1073/pnas.2023328119; |
Montllor-Albalate C, Kim H, Thompson AE, Jonke AP, Torres MP, Reddi AR, Sod1 integrates oxygen availability to redox regulate NADPH production and the thiol redoxome. Proc Natl Acad Sci U S A, 119(1):(2022) [pubmed] |
Keyword List
submitter keyword: pentose phosphate pathway, superoxide dismutase,oxygen sensing, redox signailng, glycolysis |
Contact List
Matthew Phillip Torres |
contact affiliation | School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA 30332 |
contact email | mtorres35@gatech.edu |
lab head | |
Matthew Torres |
contact affiliation | Georgia Institute of Technology |
contact email | mtorres35@gatech.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD030372
- Label: PRIDE project
- Name: Sod1 Integrates Oxygen Availability to Redox Regulate NADPH Production and the Thiol Redoxome