PXD030369

PXD030369 is an original dataset announced via ProteomeXchange.

Title	Omics-based analysis of honey bee (Apis mellifera) response to Varroa sp. interaction reveals changes impairing the winter bee generation
Description	Extensive annual losses of honey bees (Apis mellifera L.) represent a global problem for agriculture and biodiversity. The parasitic mite Varroa destructor in association with viral co-infections plays a key role in this phenomenon; however, the precise mechanisms are still unclear. We employed a unique combination of transcriptomic, proteomic, metabolomic, and functional analyses to elucidate the effects of Varroa parasitisation. We focused on complex differences between parasitised and unparasitised ten-days old honey bee workers collected from identical colonies before overwintering. Honey bees exposed to mite parasitation during their development revealed alterations in transcriptome and proteome related to immunity, oxidative stress, olfactory recognition, metabolism of sphingolipids and RNA regulatory mechanisms. Specifically, immune reactions and sphingolipids metabolism were strongly up-regulated in parasitised honey bees; whereas olfactory recognition and oxidative stress pathways were down-regulated compared to unparasitised bees. Additionally, the metabolomic analysis confirmed the depletion of nutrients, decreased energy stores and generally disrupted metabolism of parasitised workers, as previously reported. By virtue of comprehensive omics-based analysis, we define the key changes in the honey bee facing Varroa parasitism and suggest possible mechanisms underlying its detrimental effects. This study provides a theoretical basis for future efforts in efficient control strategies against Varroa mites.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_06:46:27.254.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rachel Ward
SpeciesList	scientific name: Apis mellifera (Honeybee); NCBI TaxID: 7460;
ModificationList	monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-13 09:19:54	ID requested
1	2023-06-23 01:31:27	announced
⏵ 2	2023-11-14 06:46:27	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Apis mellifera, disease, proteomics, Varroa destructor

Kevin Kavanagh
contact affiliation	Lab Head, Medical Mycology Lab
contact email	kevin.kavanagh@mu.ie
lab head
Rachel Ward
contact affiliation	Maynooth Univeristy
contact email	rachel.ward.2015@mumail.ie
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030369
     1. Label: PRIDE project
     2. Name: Omics-based analysis of honey bee (Apis mellifera) response to Varroa sp. interaction reveals changes impairing the winter bee generation