PXD030353

PXD030353 is an original dataset announced via ProteomeXchange.

Title	Exploring the molecular mechanism of dihydroartemisinin regulating spleen immunity based on proteomics and phosphoproteomic
Description	The molecular mechanisms through which dihydroartemisinin (DHA) induces immunomodulation in vivo remain unclear. Herein, DHA-regulated proteins and their phosphorylation profiles were identified and characterized, in the spleens of mice, using proteomics and phosphoproteomic approaches. We found that DHA upregulates proliferation-associated protein (cyclin-dependent kinases, Ki67, and mini-chromosome maintenance and proliferating cell nuclear antigen) expression via modulation of mitogen-activated protein kinase (MAPK) - activator protein 1 (AP-1) signaling pathway activity. In addition, DHA promoted the proliferation of CD4 T naïve, proliferated CD25+CD4+ T-cells, and BrdU+ interferon (IFN)-γ-producing CD8+ T cells. These predicted mechanisms of action were then validated in a Plasmodium berghei ANKA-infected mouse model of malaria and a cyclophosphamide-induced immunosuppression model. Collectively, the findings of this study, for the first time, provide robust evidence that DHA induced the expansion of the splenic T-cell pool through MAPK-AP1 signaling. The data provided in-depth knowledge in the mechanism of DHA-mediated immunomodulation.
HostingRepository	PRIDE
AnnounceDate	2022-05-29
AnnouncementXML	Submission_2022-05-29_07:51:06.981.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	qilong li
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-13 03:05:21	ID requested
⏵ 1	2022-05-29 07:51:07	announced

Dataset with its publication pending

submitter keyword: Dihydroartemisinin, Immunomodulatory activity, Proteomics, Phosphoproteomic

Qijun Chen
contact affiliation	Shenyang Agriculture University
contact email	qijunchen759@syau.edu.cn
lab head
qilong li
contact affiliation	Shenyang agriculture university
contact email	619827117@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030353
     1. Label: PRIDE project
     2. Name: Exploring the molecular mechanism of dihydroartemisinin regulating spleen immunity based on proteomics and phosphoproteomic