PXD030350

PXD030350 is an original dataset announced via ProteomeXchange.

Title	Small extracellular vesicles from young plasma reverse age-related functional declines by improving mitochondrial energy metabolism
Description	Aging and the chronic diseases associated with aging have become a great burden to modern society. Recent animal studies on heterochronic parabiosis have revealed that young blood has a powerful rejuvenating effect on aged tissues, but which components of the young blood are responsible for the rejuvenating effects remains unclear. In this study, we found that small extracellular vesicles (sEVs) purified from the plasma of young mice could counteract pre-existing aging at the molecular, mitochondrial, cellular and physiological levels. In detail, injection of young sEVs into aged mice extended lifespan, attenuated senescent phenotypes and mitigate age-associated impairments on various tissues (hippocampus, muscle, heart, testis, bone, etc). Mechanistical studies using iTRAQ-based quantitative proteomic analyses combined with GO term cluster revealed that the altered proteomes in aged tissues of young sEVs-treated mice were specifically related to their roles in regulating cellular senescence, metabolic process, epigenetic modification, genomic stability, etc, which are the cardinal features associated with aging. Particularly, the sEVs derived from young mice and young human donors could stimulate PGC-1α (a master regulator of mitochondrial biogenesis and energy metabolism) expression in vitro and in vivo through their rejuvenating miRNA cargos, thereby facilitating mitochondrial regeneration and counteracting mitochondrial deficits in aged tissues. Taken together, this study demonstrates that young sEVs can reverse degenerative changes and age-related dysfunctions through stimulating PGC-1α expression and regenerating intact mitochondria.
HostingRepository	PRIDE
AnnounceDate	2024-03-31
AnnouncementXML	Submission_2024-03-31_05:30:33.589.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jingzi Zhang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-13 02:59:51	ID requested
⏵ 1	2024-03-31 05:30:33	announced

10.1038/S43587-024-00612-4;

submitter keyword: miRNA., young blood, PGC-1α,Aging, mitochondria, rejuvenation

Chen Xi
contact affiliation	Nanjing University School of Life Sciences
contact email	xichen@nju.edu.cn
lab head
Jingzi Zhang
contact affiliation	Medical School of Nanjing University
contact email	zhangjingzi@nju.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/03/PXD030350

PRIDE project URI

• PRIDE
  1. PXD030350
     1. Label: PRIDE project
     2. Name: Small extracellular vesicles from young plasma reverse age-related functional declines by improving mitochondrial energy metabolism