Posttraumatic stress disorder (PTSD) is a serious stress disorder that occurs in individuals who have experienced major events. The underlying pathological mechanisms of PTSD are complex, and the related predisposing factors are still not fully understood. In this study, the proteomics and metabolomics characteristics between male the C57BL/6 normal group mice and a PTSD mice model group were analyzed using label-free quantitative proteomics and untargeted metabolomics. Differential expression analysis showed that 27 proteins were significantly differentially expressed between the two groups. Bioinformatics analysis revealed that the differentiated proteins were mostly enriched in: small molecule binding, transporter activity, extracellular region, extracellular space, endopeptidase activity, zymogen activation, hydrolase activity, proteolysis, peptidase activity, sodium channel regulator activity.