PXD030335

PXD030335 is an original dataset announced via ProteomeXchange.

Title	Identification of TBBPA-degraders in anaerobic bioreactor at environmentally relevant concentrations by combined protein-based stable isotope probing and metagenomics
Description	The aim of this study was to identify TBBPA-degrading organisms in a complex microbial community by a metagenome-based functional metaproteomic approach, using protein-based stable isotope probing (protein-SIP). Firstly, the degradation kinetics were evaluated in order to simulate the decrease of residual mass of the labelled compound based on experimental data. In sequence, a metagenome was generated, and biomass was collected in different time-points for protein-SIP in incubations with 13C-TBBPA. This approach allowed for the identification organisms assimilating labelled carbon from the cometabolic degradation of a micropollutant.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:54:11.348.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jan Struckmann Poulsen
SpeciesList	scientific name: anaerobic digester metagenome; NCBI TaxID: 1263854;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-12 14:03:56	ID requested
1	2022-09-12 03:48:04	announced
⏵ 2	2023-11-14 08:54:11	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: anaerobic digestion,Stable isotope probing, Tetrabromobisphenol A, metaproteomics

Jeppe Lund Nielsen
contact affiliation	aDepartment of Chemistry and Bioscience, Aalborg University, Aalborg East, Denmark
contact email	jln@bio.aau.dk
lab head
Jan Struckmann Poulsen
contact affiliation	Aalborg University
contact email	jsp@bio.aau.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD030335

PRIDE project URI

• PRIDE
  1. PXD030335
     1. Label: PRIDE project
     2. Name: Identification of TBBPA-degraders in anaerobic bioreactor at environmentally relevant concentrations by combined protein-based stable isotope probing and metagenomics