PXD030331

PXD030331 is an original dataset announced via ProteomeXchange.

Title	Identification of novel glycan modification on S-layer protein from Methanoculleus Marisnigri
Description	Mass spectrometry and NMR were used to characterize the N- and O-glycan modifications expressed by Methanoculleus marisnigri, a mesophilic methanogen from the Order Methanomicrobiales. The S-layer protein was identified as a PGF-CTERM sorting domain-containing protein encoded by MEMAR_RS02690 and is both N- and O-glycosylated. Two N-glycans were identified by NMR and MS analysis: a trisaccharide alpha-GlcNAc-4-beta-GlcNAc3NGaAN-4-beta-Glc-Asn where the 2nd residue is 2-N-acetyl, 3-N-glyceryl-glucosamide and a disaccharide beta-GlcNAc3NAcAN-4-beta-Glc-Asn, where the terminal residue is 2,3 di-N-acetyl-glucosamide. The S-layer protein is also extensively modified in the threonine-rich region near the C-terminus with O-glycans composed exclusively of hexoses. While the S-layer protein has a predicted PGF-CTERM processing site, no evidence of a truncated and lipidated C-terminus, the expected product of processing by an archaeosortase, was found. This is the first report of N- and O-glycosylation in an archaeon from the Order Methanomicrobiales.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:44:58.855.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD030331
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	John Kelly
SpeciesList	scientific name: Archaea; NCBI TaxID: NCBITaxon:2157; scientific name: Methanoculleus marisnigri JR1; NCBI TaxID: 368407;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse; Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-12 05:27:27	ID requested
1	2023-03-23 03:51:13	announced
⏵ 2	2023-11-14 08:44:59	announced	2023-11-14: Updated project metadata.

10.6019/PXD030331;

Kelly J, Vinogradov E, Robotham A, Tessier L, Logan SM, Jarrell KF, Characterizing the N- and O-linked glycans of the PGF-CTERM sorting domain-containing S-layer protein of Methanoculleus marisnigri. Glycobiology, 32(7):629-644(2022) [pubmed]

submitter keyword: O-Glycosylation, S-layer protein,Archaea, N-Glycosylation, Post-translational modification

John Kelly
contact affiliation	Human Heath Therapeutics, National Research Council of Canada, 100 Sussex Drive, Ottawa, Ontario, Canada K1A 0R6
contact email	John.Kelly@nrc-cnrc.gc.ca
lab head
John Kelly
contact affiliation	Human Health Therapeutics, National Research Council of Canada
contact email	John.Kelly@nrc-cnrc.gc.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030331
     1. Label: PRIDE project
     2. Name: Identification of novel glycan modification on S-layer protein from Methanoculleus Marisnigri