PXD030325

PXD030325 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis distinguishes extracellular vesicles produced by cancerous versus healthy pancreatic organoids
Description	Extracellular vesicles (EVs) are produced and released by both healthy and malignant cells and bear markers indicative of ongoing biological processes. In the present study we utilized high resolution flow cytometry to detect EVs in the plasma of patients with pancreatic ductal adenocarcinoma (PDAC) and in the supernatants of PDAC and healthy control (HC) pancreatic organoid cultures. Using ultrafiltration and size exclusion chromatography, PDAC and HC pancreatic organoid EVs were isolated for mass spectrometry analysis. Proteomic and functional analysis showed a striking distinction in that EV proteins profiled in pancreatic cancer organoids were involved in vesicular transport and tumorigenesis while EV proteins in healthy organoids were involved in cellular homeostasis. Thus, the most abundant proteins identified in either case represented non-overlapping cellular programs. Tumor-promoting candidates LAMA5, SCDB1 and TENA were consistently upregulated in PDAC EVs. Validation of specific markers for PDAC EVs versus healthy pancreatic EVs will provide the biomarkers and enhanced sensitivity necessary to monitor early disease or disease progression, with or without treatment. Moreover, disease-associated changes in EV protein profiles provide an opportunity to investigate alterations in cellular programming with disease progression.
HostingRepository	PRIDE
AnnounceDate	2022-04-04
AnnouncementXML	Submission_2022-04-03_18:09:21.905.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Phillip Wilmarth
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-12 05:11:12	ID requested
⏵ 1	2022-04-03 18:09:22	announced

Buenafe AC, Dorrell C, Reddy AP, Klimek J, Marks DL, Proteomic analysis distinguishes extracellular vesicles produced by cancerous versus healthy pancreatic organoids. Sci Rep, 12(1):3556(2022) [pubmed]

submitter keyword: pancreatic ductal adenocarcinoma, PDAC, extracellular vesicles, high resolution flow cytometry, mass spectrometry, EV markers, biomarkers

Dr. Abigail C. Buenafe
contact affiliation	Papé Family Pediatric Research Institute Oregon Health & Science University 3181 SW Sam Jackson Park Road Portland, Oregon 97239, USA
contact email	buenafea@ohsu.edu
lab head
Phillip Wilmarth
contact affiliation	OHSU
contact email	wilmarth@ohsu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030325
     1. Label: PRIDE project
     2. Name: Proteomic analysis distinguishes extracellular vesicles produced by cancerous versus healthy pancreatic organoids