PXD030317

PXD030317 is an original dataset announced via ProteomeXchange.

Title	Revisiting the role of beta-tubulin in Drosophila development: beta-tubulin60D is not an essential gene, and its novel Pin1 allele has a tissue-specific dominant-negative impact
Description	Diversity in cytoskeleton organization and function may be achieved through alternative tubulin isotypes and by a variety of post-translational modifications. The Drosophila genome contains five different β-tubulin paralogs, which may play an isotype tissue-specific function in vivo. One of these genes, the beta-tubulin60D gene, which is expressed in a tissue-specific manner, was found to be essential for fly viability and fertility. To further understand the role of the beta-tubulin60D gene, we generated new beta-tubulin60D null alleles (beta-tubulin60DM) using the CRISPR/Cas9 system and found that the homozygous flies were viable and fertile. Moreover, using a combination of genetic complementation tests, rescue experiments, and cell biology analyses, we identified Pin1, an unknown dominant mutant with bristle developmental defects, as a dominant-negative allele of beta-tubulin60D. We also found a missense mutation in the Pin1 mutant that results in an amino acid replacement from the highly conserved glutamate at position 75 to lysine (E75K). Analyzing the β-tubulin structure suggests that this E75K alteration destabilizes the alpha-helix structure and may also alter the GTP-Mg2+ complex binding capabilities. Our results revisited the credence that beta-tubulin60D is required for fly viability and revealed for the first time in Drosophila, a novel dominant-negative function of missense beta-tubulin60D mutation in bristle morphogenesis
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_06:20:05.223.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tamar Ziv
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-11 15:39:50	ID requested
⏵ 1	2022-02-17 06:20:06	announced

Krishnan RK, Halachmi N, Baskar R, Bakhrat A, Zarivach R, Salzberg A, Abdu U, Allele has a Tissue-Specific Dominant-Negative Impact. Front Cell Dev Biol, 9():787976(2021) [pubmed]

submitter keyword: pin1 development tubulin

Uri Abdu
contact affiliation	Department of Life Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel
contact email	abdu@bgu.ac.il
lab head
Tamar Ziv
contact affiliation	Technion
contact email	tamarz@technion.ac.il
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030317
     1. Label: PRIDE project
     2. Name: Revisiting the role of beta-tubulin in Drosophila development: beta-tubulin60D is not an essential gene, and its novel Pin1 allele has a tissue-specific dominant-negative impact