PXD030309

PXD030309 is an original dataset announced via ProteomeXchange.

Title	Proteome-wide cellular thermal shift assay reveals novel crosstalk between brassinosteroid and auxin signaling
Description	Despite the growing interest in using chemical genetics in plant research, small-molecule target identification remains a major challenge. The cellular thermal shift assay coupled with high-resolution massspectrometry (CETSA MS) that monitors changes in the thermal stability of proteins caused by their interactions with small molecules, other proteins, or post-translational modifications allows the identification of drug targets, or the study of protein-metabolite and protein-protein interactions mainly in mammalian cells. To showcase the applicability of this method in plants, we applied CETSA MS to intact Arabidopsis thaliana cells and identified the thermal proteome of the plant-specific glycogen synthase kinase 3 (GSK3) inhibitor, bikinin. A comparison between the thermal- and the phospho-proteomes of bikinin revealed the auxin efflux carrier PIN-FORMED1 (PIN1) as a novel substrate of the Arabidopsis GSK3s that negatively regulate the brassinosteroid signaling. We established that PIN1 phosphorylation by the GSK3s is essential for maintaining its intracellular polarity that is required for auxin-mediated regulation of vascular patterning in the leaf thus, revealing a novel crosstalk between brassinosteroid and auxin signaling.
HostingRepository	PRIDE
AnnounceDate	2022-05-31
AnnouncementXML	Submission_2022-05-31_06:33:39.803.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jarne Pauwels
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-11 15:26:41	ID requested
⏵ 1	2022-05-31 06:33:40	announced

Lu Q, Zhang Y, Hellner J, Giannini C, Xu X, Pauwels J, Ma Q, Dejonghe W, Han H, Van de Cotte B, Impens F, Gevaert K, De Smet I, Friml J, Molina DM, Russinova E, unexpected cross-talk between brassinosteroid and auxin signaling. Proc Natl Acad Sci U S A, 119(11):e2118220119(2022) [pubmed]

submitter keyword: Cellular thermal shift assay, chemical genetics, brassinosteroids, auxin

Eugenia Russinova
contact affiliation	Department of Plant Biotechnology and Bioinformatics, Ghent University; 9052 Ghent, Belgium; Center for Plant Systems Biology, VIB; 9052 Ghent, Belgium
contact email	Eugenia.Russinova@psb.vib-ugent.be
lab head
Jarne Pauwels
contact affiliation	VIB-Ugent
contact email	jarne.pauwels@vib-ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD030309

PRIDE project URI

• PRIDE
  1. PXD030309
     1. Label: PRIDE project
     2. Name: Proteome-wide cellular thermal shift assay reveals novel crosstalk between brassinosteroid and auxin signaling