PXD030302

PXD030302 is an original dataset announced via ProteomeXchange.

Title	NuA4 and H2A.Z control environmental responses and autotrophic growth in Arabidopsis
Description	Nucleosomal acetyltransferase of H4 (NuA4) is an essential transcriptional coactivator in eukaryotes, but remains poorly characterized in plants. Here, we describe Arabidopsis homologs of the NuA4 scaffold proteins Enhancer of Polycomb-Like-1 (AtEPL1) and Esa1- Associated Factor 1 (AtEAF1). Loss of AtEAF1 results in inhibition of growth and chloroplast development. These effects are stronger in the Atepl1 mutant and are further enhanced by loss of Golden2-Like (GLK) transcription factors, suggesting that NuA4 activates nuclear plastid genes alongside GLK. We demonstrate that AtEPL1 is necessary for nucleosomal acetylation of histones H4 and H2A.Z by NuA4 in vitro. These chromatin marks are diminished genome-wide in Atepl1, while another active chromatin mark, H3K9 acetylation (H3K9ac), is locally enhanced. Expression of many chloroplast-related genes depends on NuA4, as they are downregulated with loss of H4ac and H2A.Zac. Finally, we demonstrate that NuA4 promotes H2A.Z deposition and by doing so prevents spurious activation of stress response genes.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:30:43.011.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD030302
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Dominique Eeckhout
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-11 15:14:56	ID requested
1	2021-12-20 08:15:33	announced
⏵ 2	2024-10-22 05:30:43	announced	2024-10-22: Updated project metadata.

10.6019/PXD030302;

submitter keyword: NuA4 / EAF1 / EPL1 / H4 acetylation / H2A.Z acetylation / H2A.Z deposition / photosynthesis / stress response

Piotr A. Ziolkowski
contact affiliation	Laboratory of Genome Biology, Institute of Molecular Biology and Biotechnology, Adam 8 Mickiewicz University, 61-614 Poznan, Poland
contact email	pzio@amu.edu.pl
lab head
Dominique Eeckhout
contact affiliation	Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, B-9052, Belgium VIB Center for Plant Systems Biology, Ghent, B-9052, Belgium
contact email	Dominique.Eeckhout@psb.ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/12/PXD030302

PRIDE project URI

• PRIDE
  1. PXD030302
     1. Label: PRIDE project
     2. Name: NuA4 and H2A.Z control environmental responses and autotrophic growth in Arabidopsis