PXD030296

PXD030296 is an original dataset announced via ProteomeXchange.

Title	Mammalian CDC14 control differentiation from pluripotent cells by modulating UTF1 activity on bivalent promoters
Description	CDC14 phosphatases are critical components of the cell cycle machinery that drives exit from mitosis in yeast. However, the two mammalian paralogs, CDC14A and CDC14B, are dispensable for cell cycle progression or exit, and their function remains unclear. By generating a double Cdc14a; Cdc14b-null mouse model, we report here that CDC14 phosphatases control cell differentiation in pluripotent cells and their absence results in deficient development of the neural system. Lack of CDC14 impairs neural differentiation from embryonic stem cells (ESCs) accompanied by deficient induction of genes controlled by bivalent promoters. During ESC differentiation, CDC14 directly dephosphorylates and destabilizes Undifferentiated embryonic Transcription Factor 1 (UTF1), a critical regulator of stemness. In the absence of CDC14, increased UTF1 levels prevent the firing of bivalent promoters, resulting in defective induction of the transcriptional programs required for differentiation. These results suggest that mammalian CDC14 phosphatases function during the terminal exit from the cell cycle by modulating the transition from the pluripotent to the differentiated chromatin state, at least partially by controlling chromatin dynamics and transcription in a UTF1-dependent manner.
HostingRepository	PRIDE
AnnounceDate	2022-10-31
AnnouncementXML	Submission_2022-10-31_05:12:04.720.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	EduardoZarzuela
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; deamidated residue
Instrument	Q Exactive HF; Q Exactive HF-X; Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-10 02:38:18	ID requested
⏵ 1	2022-10-31 05:12:05	announced

Dataset with its publication pending

submitter keyword: mESCs, UTF1,CDC14

Javier MuñozPeralta
contact affiliation	Spanish National Cancer Research Centre (CNIO)
contact email	javier.munozperalta@osakidetza.eus
lab head
EduardoZarzuela
contact affiliation	Centro Nacional de Investigaciones Oncológicas
contact email	eduzarfer@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030296
     1. Label: PRIDE project
     2. Name: Mammalian CDC14 control differentiation from pluripotent cells by modulating UTF1 activity on bivalent promoters