PXD030293

PXD030293 is an original dataset announced via ProteomeXchange.

Title	Fasciola hepatica cathepsin L zymogens: immuno-proteomic evidence for highly immunogenic zymogen-specific conformational epitopes to support diagnostics development
Description	We conducted immuno-proteomic investigations of recombinant and native procathepsins ((r)FhpCL1) and counterpart polyclonal antibodies to a recombinant mutant procathepsin L (anti-rFhΔpCL1). Proteomics including gel electrophoresis and liquid chromatography tandem mass spectrometry were used for formal identification and in the determination of sample compositions. We have confirmed recombinant and native cathepsin L zymogens contain conserved, highly antigenic epitopes that are conformationally dependent.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:54:24.003.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD030293
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Clare Collett
SpeciesList	scientific name: Fasciola hepatica (Liver fluke); NCBI TaxID: 6192;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	6550 iFunnel Q-TOF LC/MS; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-10 02:26:25	ID requested
1	2022-07-19 03:07:41	announced
⏵ 2	2023-11-14 08:54:24	announced	2023-11-14: Updated project metadata.

10.6019/PXD030293;

submitter keyword: triclabendazole., recombinant,Fasciolosis, cathepsin, diagnostics

Professor Peter Brophy
contact affiliation	Veterinary Parasitology Group, Institute of Biological Environmental and Rural Sciences (IBERS), Aberystwyth University, Penglais, Aberystwyth, Ceredigion, SY23 3FL
contact email	pmb@aber.ac.uk
lab head
Clare Collett
contact affiliation	Aberystwyth University
contact email	clare.f.collett@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030293
     1. Label: PRIDE project
     2. Name: Fasciola hepatica cathepsin L zymogens: immuno-proteomic evidence for highly immunogenic zymogen-specific conformational epitopes to support diagnostics development