PXD030286

PXD030286 is an original dataset announced via ProteomeXchange.

Title	Identification of microproteins in Hep3B cells at different cell cycle stages
Description	Microproteins are generated from small open reading frames (sORFs) and turn out to play various vital biological functions. As an essential biological event of eukaryotic cells, the cell cycle is involved in cell replication and division. For such a highly regulated event, microproteins associated with cell cycle regulation remained unclarified. Utilizing a combination of bottom-up and top-down proteomics, we analyzed microproteins at specific cell cycle stages of Hep3B cells. A total of 657 microproteins were identified under three cell cycle stages, including 151 in the G0/G1 stage, 163 in the S stage, and 132 in the G2/M stage. The annotation of these microproteins showed their cell cycle-specific functions, such as translation, nuclear assembly, chromatin organization, G2/M transition of the mitotic cell cycle. Meanwhile, more than 50 % of identified microproteins were ncRNA encoded. These non-annotated novel microproteins contain several function domains, such as nucleoside diphosphate kinase (NDK) domain, high mobility group (HMG) domain, and DNA-binding domain. That suggested the potential functions of these novel microproteins in specific cell cycle stages. This study presented a large-scale profile of microproteins at different cell cycle stages from Hep3B and may provide new perspectives on the regulation mechanism of the cell cycle.
HostingRepository	iProX
AnnounceDate	2021-12-09
AnnouncementXML	Submission_2022-10-10_21:41:44.389.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bing Li
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-10 00:27:46	ID requested
1	2021-12-10 00:40:15	announced
⏵ 2	2022-10-10 21:41:45	announced	2022-10-11: Update publication information.

Wang Z, Pan N, Yan J, Wan J, Wan C, . J Proteome Res, 21(4):1114-1123(2022) [pubmed]

Pan N, Wang Z, Wang B, Wan J, Wan C, Mapping Microproteins and ncRNA-Encoded Polypeptides in Different Mouse Tissues. Front Cell Dev Biol, 9():687748(2021) [pubmed]

submitter keyword: cell cycle, Hep3B, microprotein, DNA-binding domain, ncRNA

Cuihong Wan
contact affiliation	a. School of Life Sciences
contact email	ch_wan@mail.ccnu.edu.cn
lab head
Bing Li
contact affiliation	Central China Normal University
contact email	2298267977@qq.com
dataset submitter

iProX dataset URI