PXD030281

PXD030281 is an original dataset announced via ProteomeXchange.

Title	Drosophila early embryos LC-MSMS
Description	Cytoplasmic polyadenylation of mRNAs promotes their translation in a wide variety of biological contexts. The conserved RNA-binding protein family CPEB has been shown to mediate canonical cytoplasmic polyadenylation of target transcripts. We have previously reported evidence for RNA-interference factor Dicer-2 as a component of a non-canonical complex, that operates independent of CPEB in Drosophila. In this study, we investigated Dicer-2 mRNA targets and protein co-factors in cytoplasmic polyadenylation. We identified hundreds of novel Dicer-2 target transcripts, ~50% of which were previously found as targets of the cytoplasmic poly(A) polymerase Wispy, suggesting widespread roles of Dicer-2 in cytoplasmic polyadenylation. Large-scale immunoprecipitation and mass spectrometry revealed Ataxin-2 and Twenty-four among the high-confidence interactors of Dicer-2. Functional analysis indicates that both factors form an RNA-independent complex with Dicer-2 and are required for cytoplasmic polyadenylation of Dicer-2 targets. Our results reveal the composition of a novel cytoplasmic polyadenylation complex that operates during Drosophila early embryogenesis.
HostingRepository	PRIDE
AnnounceDate	2022-08-30
AnnouncementXML	Submission_2022-08-30_02:32:59.737.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hima Priyanka Nadimpalli
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-09 03:32:19	ID requested
⏵ 1	2022-08-30 02:33:00	announced

Dataset with its publication pending

submitter keyword: Drosophila, early embryos, Dicer-2, LC-MSMS

Fátima, Gebauer
contact affiliation	Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Barcelona, Spain. University of Pompeu Fabra (UPF), Barcelona, Spain
contact email	fatima.gebauer@crg.eu
lab head
Hima Priyanka Nadimpalli
contact affiliation	Center for Integrative Genomics, University of Lausanne
contact email	himapriyanka.n@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD030281

PRIDE project URI

• PRIDE
  1. PXD030281
     1. Label: PRIDE project
     2. Name: Drosophila early embryos LC-MSMS