PXD030280

PXD030280 is an original dataset announced via ProteomeXchange.

Title	DNR pulse treatment of primary AML
Description	Primary human AML bone marrow sample (newly diagnosed, prior to treatment initation) was obtained from donor after consent and AML blasts were isolated by standard Ficoll centrifugation. AML blasts were pulse-treated with daunorubicine (100 nM, 200 nM, 400 nM) or DMSO control for 1 hour, followed by wash-out and short-term cell culture to allow cellular responses to develop. 20,000 cells were analysed by LC-MS3, and 5,000 cells were subjected to flow cytometry apoptosis assay.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:53:55.270.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sebastian Koschade
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-09 03:28:26	ID requested
1	2022-10-14 19:25:53	announced
⏵ 2	2023-11-14 08:53:55	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: AML, DNR

Christian Muench
contact affiliation	Institute of Biochemistry II, Goethe University, Frankfurt, Germany
contact email	ch.muench@em.uni-frankfurt.de
lab head
Sebastian Koschade
contact affiliation	Department of Medicine, Hematology/Oncology, University Hospital Frankfurt, Goethe University, Frankfurt, Germany & Institute of Biochemistry II, Faculty of Medicine, Goethe University, Frankfurt, Germany
contact email	koschade@med.uni-frankfurt.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD030280

PRIDE project URI

• PRIDE
  1. PXD030280
     1. Label: PRIDE project
     2. Name: DNR pulse treatment of primary AML