PXD030277

PXD030277 is an original dataset announced via ProteomeXchange.

Title	Glycoproteomics of human THP-1-derived M0, M1 and M2 macrophages
Description	In this study, we used human THP-1-derived macrophages as an immune cell model, and systematically performed glycoproteomics of three subtypes of macrophages by StrucGP.To study the N‑Glycoproteomic of them, the intact glycopeptides were first enriched and identified using triplicate mass spectrometry (MS)-based glycoproteomic approaches, then StrucGP was used for subsequent analysis. In total three subtypes of macrophages, these intact glycopeptides consist of 253 N-linked glycan structures at 652 unique glycosites from 372 N-glycoproteins, along with their PSM information. A total of 135, 163, and 201 N-glycan structures were identified from M0, M1, and M2 macrophages, respectively.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:29:58.512.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pengfei Li
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-09 03:10:49	ID requested
1	2023-10-18 18:43:58	announced
⏵ 2	2023-11-14 08:29:58	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: macrophages, Glycoproteomics, LC-MS/MS

Pengfei Li
contact affiliation	College of Life Sciences, Northwest University, Xi'an, Shaanxi province, China
contact email	pengfeilibio@163.com
lab head
Pengfei Li
contact affiliation	College of Life Sciences, Northwest University, Xi'an, China
contact email	pengfeilibio@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD030277

PRIDE project URI

• PRIDE
  1. PXD030277
     1. Label: PRIDE project
     2. Name: Glycoproteomics of human THP-1-derived M0, M1 and M2 macrophages