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PXD030275

PXD030275 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQuantitative Proteomics Revealed the Changes of Pharmacodynamic Network of Bugu Shengsui Decoction Promoting Osteoblasts Proliferation
DescriptionInsufficient osteogenesis is greatly essential to osteoporosis. Bugu Shengsui Decoction, a compound formula for osteoporosis, has significant clinical effects in the treatment of osteoporosis. Yet the detailed mechanisms are unclear. In this study, we first evaluated the pharmacological effects of Bugu Shengsui Decoction on bone metabolism, bone mineral density, bone morphology and biomechanics in ovariectomized rats. It also clarified the promoting effect of Bugu Shengsui Decoction on the proliferation and differentiation of osteoblasts. Based on the above results, we conducted quantitative proteomics research and a series of validation experiments, the results showed that PI3K-AKT signaling pathway and targets, such as Fn1, Col1a1, Col1a2, Col6a1, Col6a2, Col6a3, Rac1, Hsp90ab1, Hsp90b1, Ywhaz and Gnb2, were most relevant to the anti-osteoporosis efficacy of BGSSD. Summarily, our discoveries certify that Bugu Shengsui Decoction is an effective treatment for osteoporosis via PI3K-AKT.
HostingRepositoryPRIDE
AnnounceDate2024-10-07
AnnouncementXMLSubmission_2024-10-07_13:44:04.601.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBaoyu Qi
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList2x(13)C,6x(2)H labeled dimethylated L-arginine; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-12-09 02:59:10ID requested
12024-10-07 13:44:05announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Osteoporosis, Osteoblasts, Bugu Shengsui Decoction, Proliferation, Quantitative Proteomics, PI3K-Akt, Pharmacodynamic Network
Contact List
Jianye Dai
contact affiliationSchool of Pharmacy, Lanzhou University, Lanzhou, China
contact emaildaijy@lzu.edu.cn
lab head
Baoyu Qi
contact affiliationWangjing Hospital, China Academy of Chinese Medical Sciences
contact emailqiby325@163.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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