PXD030263

PXD030263 is an original dataset announced via ProteomeXchange.

Title	In-depth assembly of organ and development dissected Picrorhiza kurroa proteome map using mass spectrometry
Description	Background Picrorhiza kurroa, being a rich source of phytochemicals, is a promising higher altitude medicinal herb of Himalaya. The medicinal potential is attributed to picrosides i.e. iridoid glycosides, which synthesized in organ-specific manner through highly complex pathways. Here, we present a large-scale proteome reference map of P. kurroa, consisting of four morphologically differentiated organs and two developmental stages. Results We were able to identify 5186 protein accessions (FDR<1%) providing a deep coverage of protein abundance array, spanning around six orders of magnitude. Most of the identified proteins are associated with metabolic processes, response to abiotic stimuli and cellular processes. Organ specific sub-proteomes highlights organ specialized functions that would offer insights to explore tissue profile for specific protein classes. With reference to P. kurroa development, vegetative phase is enriched with growth related processes, however generative phase harvests more energy in secondary metabolic pathways. Furthermore, stress-responsive proteins, RBPs and PTMs, particularly phosphorylation and ADP-ribosylation play an important role in P. kurroa adaptation to alpine environment. The proteins involved in the synthesis of secondary metabolites are well represented in P. kurroa proteome. The phytochemical analysis revealed that marker compounds were highly accumulated in rhizome and overall, during the late stage of development. Conclusions This report represents first extensive proteomic description of organ and developmental dissected P. kurroa, providing a platform for future studies related to stress tolerance and medical applications.
HostingRepository	PRIDE
AnnounceDate	2022-02-15
AnnouncementXML	Submission_2022-02-15_09:05:04.351.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rajiv Kumar
SpeciesList	scientific name: Picrorhiza kurrooa; NCBI TaxID: 195120;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	6560 Q-TOF LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-08 12:47:46	ID requested
⏵ 1	2022-02-15 09:05:05	announced

Kumari M, Pradhan UK, Joshi R, Punia A, Shankar R, Kumar R, In-depth assembly of organ and development dissected Picrorhiza kurroa proteome map using mass spectrometry. BMC Plant Biol, 21(1):604(2021) [pubmed]

submitter keyword: Picrorhiza kurroa, Proteome, PTMs, RBPs, Picrosides, Metabolites

RAJIV KUMAR
contact affiliation	BIOTECHNOLOGY DIVISION, CSIR-IHBT, PO BOX 6,PALAMPUR, HP, INDIA, 176061
contact email	rajiv@ihbt.res.in
lab head
Rajiv Kumar
contact affiliation	CSIR-IHBT PALAMPUR, HP, INDIA
contact email	rajiv@ihbt.res.in
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030263
     1. Label: PRIDE project
     2. Name: In-depth assembly of organ and development dissected Picrorhiza kurroa proteome map using mass spectrometry