PXD030258 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mitochondrial microproteins link metabolic cues to respiratory chain biogenesis |
Description | Electron transport chain (ETC) biogenesis is tightly coupled to energy levels and availability of ETC subunits. Coenzyme Q: cytochrome c oxidoreductase (complex III or CIII) occupies a central position in the ETC, receiving electrons from diverse fuel sources to control the ubiquinol:ubiquinone (CoQH2/CoQ) ratio. As such, CIII is an attractive node for controlling ETC biogenesis during metabolic stress. Here, we report the discovery of mammalian CoOrdinator of Mitochondrial CYTB or “COM” complexes that regulate CIII biogenesis in a step-wise fashion in response to nutrient and nuclear-encoded ETC subunit availability. The COMA complex, consisting of UQCC1/2 and the membrane anchor C16ORF91 (UQCC4), facilitates the translation of CIII enzymatic core subunit CYTB. Subsequently, microproteins SMIM4 and BRAWNIN, together with COMA subunits form the COMB complex that stabilizes nascent CYTB. Finally, UQCC3-containing COMC promotes CTYB maturation and association with downstream CIII subunits. This stepwise assembly enables cells to adapt to metabolic stress by increasing CIII biogenesis and inducing an integrated stress response when challenged. Furthermore, when nuclear CIII subunits are unavailable for assembly, COMB is required to chaperone nascent CYTB to prevent OXPHOS collapse. Our studies highlight CYTB synthesis as a key regulatory node of ETC biogenesis, and uncover the roles of mito-SEPs in mitochondrial homeostasis during energy stress. |
HostingRepository | PRIDE |
AnnounceDate | 2022-09-26 |
AnnouncementXML | Submission_2022-09-25_16:33:12.432.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | David Stroud |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Exploris 480; Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-12-08 12:39:45 | ID requested | |
⏵ 1 | 2022-09-25 16:33:13 | announced | |
Publication List
Liang C, Zhang S, Robinson D, Ploeg MV, Wilson R, Nah J, Taylor D, Beh S, Lim R, Sun L, Muoio DM, Stroud DA, Ho L, Mitochondrial microproteins link metabolic cues to respiratory chain biogenesis. Cell Rep, 40(7):111204(2022) [pubmed] |
Keyword List
submitter keyword: mitochondria, micropeptides, SEP, OXPHOS, metabolism |
Contact List
David Stroud |
contact affiliation | Department of Biochemistry and Pharmacology, The Bio21 Molecular Science & Biotechnology Institute, University of Melbourne, 30 Flemington Rd, Parkville Victoria 3052, Australia |
contact email | david.stroud@unimelb.edu.au |
lab head | |
David Stroud |
contact affiliation | The University of Melbourne |
contact email | david.stroud@unimelb.edu.au |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD030258
- Label: PRIDE project
- Name: Mitochondrial microproteins link metabolic cues to respiratory chain biogenesis