PXD030244

PXD030244 is an original dataset announced via ProteomeXchange.

Title	Age-dependent increase in Schmidt-Lanterman incisures and a Cadm4 associated membrane skeletal complex in fatty acid 2-hydroxylase deficient mice, a mouse model of spastic paraplegia SPG35
Description	Fatty acid 2-hydroxylase (FA2H) is responsible for the synthesis of 2-hydroxylated fatty acids, which are a building block of sphingolipids. This 2-hydroxylated sphingolipids can be found in various tissues such as brain, spinal cord, skin, testis, ovary, kidney, stomach, or intestine with a notably high occurrence in the insulating myelin sheath of neurons. Mutations in the FA2H gene can cause the neurodegenerative disease spastic paraplegia 35 (SPG35), which is also known as fatty acid hydroxylase-associated neurodegeneration. Moreover, FA2H knock-out mice largely resemble the SPG35 human disease phenotype. To further elucidate the connection between FA2H and SPG35, we performed a comparative quantitative proteome analysis of peripheral nervous system myelin of wildtype and FA2H-KO mice at different ages (6, 13, and 17 months) using isobaric labeling with tandem mass tags (TMT).
HostingRepository	PRIDE
AnnounceDate	2022-04-22
AnnouncementXML	Submission_2022-04-22_05:48:18.624.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD030244
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Robert Hardt
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; S-carboxamidoethyl-L-cysteine; monohydroxylated residue; acetylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-08 07:32:37	ID requested
⏵ 1	2022-04-22 05:48:18	announced

10.1007/S12035-022-02832-4;

submitter keyword: Isobaric labelling, TMT, myelin, PNS, mice, fatty acid hydroxylation, sphingolipids, fatty acid 2-hydroxylase, FA2H, neurodegeneration, disease model, time course, hereditary spastic paraplegia type 35, Schmidt-Lanterman incisures, sciatic nerves

Matthias Eckhardt
contact affiliation	AK Becker, Institut für Biochemie und Molekularbiologie (IBMB), Med. Fak. Universtität Bonn, Nussallee 11, 53115 Bonn, Germany
contact email	eckhardt@uni-bonn.de
lab head
Robert Hardt
contact affiliation	Institute for Biochemistry and Molecular Biology, Medical Faculty, University of Bonn, 53115 Bonn, Germany
contact email	rhardt@uni-bonn.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030244
     1. Label: PRIDE project
     2. Name: Age-dependent increase in Schmidt-Lanterman incisures and a Cadm4 associated membrane skeletal complex in fatty acid 2-hydroxylase deficient mice, a mouse model of spastic paraplegia SPG35