PXD030233

PXD030233 is an original dataset announced via ProteomeXchange.

Title	Identification of di-Boron cleavage events at Dha residues on recombinant protein in e.coli lysate
Description	Neurokinin-A (NKA) is only active when its C-terminus is amidated. B2(OH2) is thought to interact with Dha and cleave the peptide, leaving the amide on the C-terminus of the left-hand peptide. PeptideID MS/MS experiment was performed in e.coli lysate with overevpressed recombinant NKA-eH3 to prove diBoron activity in biologically relevant context. Overall analysis of the Dha generation and consumption on e.coli lysate was also performed.
HostingRepository	PRIDE
AnnounceDate	2022-05-19
AnnouncementXML	Submission_2022-05-19_10:22:06.970.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yana Demyanenko
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	amidated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-08 02:24:46	ID requested
⏵ 1	2022-05-19 10:22:07	announced

Mollner TA, Giltrap AM, Zeng Y, Demyanenko Y, Buchanan C, Oehlrich D, Baldwin AJ, Anthony DC, Mohammed S, Davis BG, -type/N2-C2 cleavage allows C-terminal protein amidation. Sci Adv, 8(14):eabl8675(2022) [pubmed]

submitter keyword: Dha, chemical cleavage, amidated C-term

Ben Davis
contact affiliation	Department of Chemistry, University of Oxford Oxford, UK; Department of Pharmacology, University of Oxford, Oxford, UK; The Rosalind Franklin Institute, Oxfordshire, UK
contact email	ben.davis@chem.ox.ac.uk
lab head
Yana Demyanenko
contact affiliation	Rosalind Franklin Institute
contact email	yana.demyanenko@rfi.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD030233

PRIDE project URI

• PRIDE
  1. PXD030233
     1. Label: PRIDE project
     2. Name: Identification of di-Boron cleavage events at Dha residues on recombinant protein in e.coli lysate