PXD030230 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Translational and Post-translational LC-MS of Naïve and Primed hESCs |
Description | Deciphering the regulatory network for human naïve and primed pluripotency is of fundamental theoretical and applicable significance. Here, by combining quantitative proteomics, phosphoproteomics and acetylproteomics analyses, we revealed RNA processing and translation as the most differentially-regulated processes between naïve and primed human embryonic stem cells (hESCs). While glycolytic primed hESCs rely predominantly on eIF4E-mediated cap-dependent pathway for protein translation, naïve hESCs with reduced mTORC1 activity are more tolerant to blockage of eIF4E-dependent translation, and their bivalent metabolism allows for translating selective mRNAs via both eIF4E-dependent and eIF4E-independent/eIF4A2-dependent pathways to form a more compact naïve proteome. Globally up-regulated proteostasis system and down-regulated post-translational modification system help to further refine and maintain the naïve proteome that is compatible with the more rapid cycling of naïve hESCs, where CDK1 plays an indispensable coordinative role. |
HostingRepository | PRIDE |
AnnounceDate | 2021-12-10 |
AnnouncementXML | Submission_2021-12-09_18:05:39.001.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Bo Kang |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue; acetylated residue |
Instrument | timsTOF Pro; Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-12-08 02:11:28 | ID requested | |
⏵ 1 | 2021-12-09 18:05:39 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: human, hESC, Naïve, Primed, LC-MS |
Contact List
Ying-Jie Wang |
contact affiliation | The First Affiliated Hospital, School of Medicine, Zhejiang University |
contact email | yingjiewang@zju.edu.cn |
lab head | |
Bo Kang |
contact affiliation | State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, School of Medicine, Zhejiang University, 310003 Hangzhou, China |
contact email | kangbo@zju.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD030230
- Label: PRIDE project
- Name: Translational and Post-translational LC-MS of Naïve and Primed hESCs