PXD030230

PXD030230 is an original dataset announced via ProteomeXchange.

Title	Translational and Post-translational LC-MS of Naïve and Primed hESCs
Description	Deciphering the regulatory network for human naïve and primed pluripotency is of fundamental theoretical and applicable significance. Here, by combining quantitative proteomics, phosphoproteomics and acetylproteomics analyses, we revealed RNA processing and translation as the most differentially-regulated processes between naïve and primed human embryonic stem cells (hESCs). While glycolytic primed hESCs rely predominantly on eIF4E-mediated cap-dependent pathway for protein translation, naïve hESCs with reduced mTORC1 activity are more tolerant to blockage of eIF4E-dependent translation, and their bivalent metabolism allows for translating selective mRNAs via both eIF4E-dependent and eIF4E-independent/eIF4A2-dependent pathways to form a more compact naïve proteome. Globally up-regulated proteostasis system and down-regulated post-translational modification system help to further refine and maintain the naïve proteome that is compatible with the more rapid cycling of naïve hESCs, where CDK1 plays an indispensable coordinative role.
HostingRepository	PRIDE
AnnounceDate	2021-12-10
AnnouncementXML	Submission_2021-12-09_18:05:39.001.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bo Kang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue
Instrument	timsTOF Pro; Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-08 02:11:28	ID requested
⏵ 1	2021-12-09 18:05:39	announced

Dataset with its publication pending

submitter keyword: human, hESC, Naïve, Primed, LC-MS

Ying-Jie Wang
contact affiliation	The First Affiliated Hospital, School of Medicine, Zhejiang University
contact email	yingjiewang@zju.edu.cn
lab head
Bo Kang
contact affiliation	State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, School of Medicine, Zhejiang University, 310003 Hangzhou, China
contact email	kangbo@zju.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/12/PXD030230

PRIDE project URI

• PRIDE
  1. PXD030230
     1. Label: PRIDE project
     2. Name: Translational and Post-translational LC-MS of Naïve and Primed hESCs