PXD030228

PXD030228 is an original dataset announced via ProteomeXchange.

Title	TMT combined with LC–MS/MS technique to detect protein markers in gastroesophageal junction adenocarcinoma
Description	Gastroesophageal junction adenocarcinoma (GEJA) is a malignant tumor located at the junction of the esophagus and stomach, and its incidence is increasing yearly, while early screening for biological markers is limited. Tandem mass tag (TMT) combined with liquid chromatography-tandem mass spectrometry (LC–MS/MS) has been used to screen for differential proteins in various cancers. In the present study, we used TMT-LC–MS/MS to screen differential proteins in GEJA and precancerous lesions and then selected proteins that met the expectation for a trend clustering analysis combined with GO and KEGG analyses to functionally annotate differential proteins in GEJA. Then, parallel reaction monitoring (PRM) and immunohistochemistry (IHC) techniques were used to verify the accuracy of the proteomics data. In conclusion, our group used proteomics technology to screen differential proteins in the progression of GEJA and explored the expression trends of differential proteins in the occurrence of the carcinogenesis process and the functional areas involved, which provided detailed information for the elucidation of the progression process, pathogenesis, early screening and screening of candidate markers of GEJA.
HostingRepository	iProX
AnnounceDate	2021-12-08
AnnouncementXML	Submission_2023-12-21_22:27:59.767.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yanna Bi
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	methylthiolated residue
Instrument	Q Exactive Plus; timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-07 21:42:50	ID requested
1	2021-12-07 21:43:05	announced
⏵ 2	2023-12-21 22:28:00	announced	2023-12-22: Update publication information.

Zheng X, Bi Y, Yang T, Zhao L, Wu M, Er L, Liu Y, Li S, Tandem mass tagging combined with liquid chromatography-tandem mass spectrometry technique to detect protein markers in gastroesophageal junction adenocarcinoma. Rapid Commun Mass Spectrom, 36(18):e9355(2022) [pubmed]

submitter keyword: Gastroesophageal junction adenocarcinoma, Precancerous lesions, TMT-LC–MS/MS, Proteome

Lianmei Zhao
contact affiliation	The Fourth Hospital of Hebei Medical University
contact email	lianmeizhmail@163.com
lab head
Yanna Bi
contact affiliation	The Fourth Hospital of Hebei Medical University
contact email	2421918544@qq.com
dataset submitter

iProX dataset URI