PXD030213

PXD030213 is an original dataset announced via ProteomeXchange.

Title	Modeling CRL4A ligase complex to predict target protein ubiquitination induced by CRBN-recruiting PROTACs
Description	PROteolysis TArgeting Chimeras (PROTACs) are bifunctional small molecules that can simultaneously recruit target protein and E3 ligase to form a ternary complex (target protein / PROTAC / E3 ligase), leading to target protein ubiquitination and degradation via the Ubiquitin-Proteasome System (UPS). PROTACs have gained increasing attention in recent years due to certain advantages over traditional therapeutic modalities enabling targeting of previously "undruggable" proteins. To better understand the mechanism of PROTAC-induced Target Protein Degradation (TPD), several computational approaches have recently been developed to study and predict ternary complex formation. However, mounting evidence suggests that ubiquitination can also be a rate-limiting step in PROTAC induced TPD. Here, we propose a structure-based computational approach to predict target protein ubiquitination induced by CRBN-based PROTACs,
HostingRepository	PRIDE
AnnounceDate	2022-05-19
AnnouncementXML	Submission_2022-05-19_07:27:34.604.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD030213
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Aman Makaju
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	ubiquitinylated lysine
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-06 04:10:58	ID requested
⏵ 1	2022-05-19 07:27:35	announced

Bai N, Riching KM, Makaju A, Wu H, Acker TM, Ou SC, Zhang Y, Shen X, Bulloch DN, Rui H, Gibson BW, Daniels DL, Urh M, Rock BM, Humphreys SC, Modeling the CRL4A ligase complex to predict target protein ubiquitination induced by cereblon-recruiting PROTACs. J Biol Chem, 298(4):101653(2022) [pubmed]

submitter keyword: PROTAC, TMT, Ubiqutination

Sara Humphreys
contact affiliation	Amgen
contact email	shumph01@amgen.com
lab head
Aman Makaju
contact affiliation	Amgen Inc
contact email	amakaju@amgen.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030213
     1. Label: PRIDE project
     2. Name: Modeling CRL4A ligase complex to predict target protein ubiquitination induced by CRBN-recruiting PROTACs