PXD030203

PXD030203 is an original dataset announced via ProteomeXchange.

Title	full length protein sequencing method based on less specific and unspecific hydrolysis strategies 2
Description	We describe a new strategy for LC-MS/MS based full length protein sequencing. Protein samples were unspecific hydrolyzed by three different methods(proteinase K, papain and microwave-assisted acid hydrolysis) to improve overlapping degree of peptides. After LC-MS/MS analysis, peptide sequences were gernated by de novo peptide sequencing program Pnovo. A new sequence assembly program, based on de brujin graph and Overlap-Layout-Consensus strategy, was desined to generate full length protein sequence using Pnovo results.
HostingRepository	PRIDE
AnnounceDate	2022-02-15
AnnouncementXML	Submission_2022-02-15_00:45:41.767.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	zhou zhong hua
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Equus caballus (Horse); NCBI TaxID: 9796;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-06 02:44:08	ID requested
⏵ 1	2022-02-15 00:45:42	announced

Dataset with its publication pending

submitter keyword: protein sequencing, unspecific hydrolysis, LC-MS/MS, de novo, MAAH

Gong Zhang
contact affiliation	Key Laboratory of function protein research of Guangdong Higer Education Institutes and MOE Key Laboratory of Tumor Molecular Biology, Institute of life and health engineering, Jinan University, Guangzhou, China
contact email	zhanggong@jnu.edu.cn
lab head
zhou zhong hua
contact affiliation	Jinan univierisy
contact email	31893592@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030203
     1. Label: PRIDE project
     2. Name: full length protein sequencing method based on less specific and unspecific hydrolysis strategies 2