PXD030181 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A dynamic and combinatorial histone code drives malaria parasite asexual and sexual development |
Description | Histone post-translational modifications (PTMs) are frequently co-occurring on the same chromatin domains or even the same molecule. It is now established that these ‘histone codes’ are the result of cross-talk between enzymes that catalyse multiple PTMs with univocal readout as compared to these PTMs in isolation. Here, we performed a comprehensive identification and quantification of histone codes of the malaria parasite, Plasmodium falciparum. We used advanced quantitative middle-down proteomics to identify combinations of PTMs in both the proliferative, asexual stages and transmissible, sexual gametocyte stages of P. falciparum. We provide an updated, high-resolution compendium of 72 PTMs on H3 and H3.3, of which 30 newly identified. Several co-existing PTMs with unique stage distinction was identified, indicating that many of these combinatorial PTMs are associated to specific stages of the parasite life cycle. We focused on the code H3R17me2K18acK23ac for its unique presence in mature gametocytes; chromatin proteomics identified a gametocyte-specific SAGA-like effector complex including the transcription factor AP2-G2 which we associated to this specific histone code, as involved in regulating gene expression in mature gametocytes. Ultimately, this study unveils previously undiscovered histone PTMs and their functional relationship with co-existing partners. These results highlight that investigating chromatin regulation in the parasite using single histone PTM assays might overlook higher order gene regulation for distinct proliferation and differentiation processes. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-07 |
AnnouncementXML | Submission_2024-10-07_13:43:43.988.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Simone Sidoli |
SpeciesList | scientific name: Plasmodium falciparum V1; NCBI TaxID: 5847; |
ModificationList | monomethylated residue; phosphorylated residue; acetylated residue; N6,N6,N6-trimethyl-L-lysine (from L-lysinium residue); N6,N6-dimethyl-L-lysine |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-12-05 04:35:03 | ID requested | |
⏵ 1 | 2024-10-07 13:43:44 | announced | |
Publication List
von Gr, ü, ning H, Coradin M, Mendoza MR, Reader J, Sidoli S, Garcia BA, Birkholtz LM, A Dynamic and Combinatorial Histone Code Drives Malaria Parasite Asexual and Sexual Development. Mol Cell Proteomics, 21(3):100199(2022) [pubmed] |
10.1016/j.mcpro.2022.100199; |
Keyword List
submitter keyword: malaria, middle-down mass spectrometry proteomics, post-translational modification, parasite, PTM,Gametocyte, histone code, Plasmodium falciparum |
Contact List
Simone Sidoli |
contact affiliation | Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY, 10461, USA |
contact email | simone.sidoli@einsteinmed.org |
lab head | |
Simone Sidoli |
contact affiliation | Albert Einstein College of Medicine |
contact email | simone.sidoli@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD030181
- Label: PRIDE project
- Name: A dynamic and combinatorial histone code drives malaria parasite asexual and sexual development