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PXD030181

PXD030181 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA dynamic and combinatorial histone code drives malaria parasite asexual and sexual development
DescriptionHistone post-translational modifications (PTMs) are frequently co-occurring on the same chromatin domains or even the same molecule. It is now established that these ‘histone codes’ are the result of cross-talk between enzymes that catalyse multiple PTMs with univocal readout as compared to these PTMs in isolation. Here, we performed a comprehensive identification and quantification of histone codes of the malaria parasite, Plasmodium falciparum. We used advanced quantitative middle-down proteomics to identify combinations of PTMs in both the proliferative, asexual stages and transmissible, sexual gametocyte stages of P. falciparum. We provide an updated, high-resolution compendium of 72 PTMs on H3 and H3.3, of which 30 newly identified. Several co-existing PTMs with unique stage distinction was identified, indicating that many of these combinatorial PTMs are associated to specific stages of the parasite life cycle. We focused on the code H3R17me2K18acK23ac for its unique presence in mature gametocytes; chromatin proteomics identified a gametocyte-specific SAGA-like effector complex including the transcription factor AP2-G2 which we associated to this specific histone code, as involved in regulating gene expression in mature gametocytes. Ultimately, this study unveils previously undiscovered histone PTMs and their functional relationship with co-existing partners. These results highlight that investigating chromatin regulation in the parasite using single histone PTM assays might overlook higher order gene regulation for distinct proliferation and differentiation processes.
HostingRepositoryPRIDE
AnnounceDate2024-10-07
AnnouncementXMLSubmission_2024-10-07_13:43:43.988.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSimone Sidoli
SpeciesList scientific name: Plasmodium falciparum V1; NCBI TaxID: 5847;
ModificationListmonomethylated residue; phosphorylated residue; acetylated residue; N6,N6,N6-trimethyl-L-lysine (from L-lysinium residue); N6,N6-dimethyl-L-lysine
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-12-05 04:35:03ID requested
12024-10-07 13:43:44announced
Publication List
von Gr, ü, ning H, Coradin M, Mendoza MR, Reader J, Sidoli S, Garcia BA, Birkholtz LM, A Dynamic and Combinatorial Histone Code Drives Malaria Parasite Asexual and Sexual Development. Mol Cell Proteomics, 21(3):100199(2022) [pubmed]
10.1016/j.mcpro.2022.100199;
Keyword List
submitter keyword: malaria, middle-down mass spectrometry proteomics, post-translational modification, parasite, PTM,Gametocyte, histone code, Plasmodium falciparum
Contact List
Simone Sidoli
contact affiliationDepartment of Biochemistry, Albert Einstein College of Medicine, Bronx, NY, 10461, USA
contact emailsimone.sidoli@einsteinmed.org
lab head
Simone Sidoli
contact affiliationAlbert Einstein College of Medicine
contact emailsimone.sidoli@gmail.com
dataset submitter
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Dataset FTP location
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