PXD030179

PXD030179 is an original dataset announced via ProteomeXchange.

Title	Cultivation impact on the protein expression profile of Mycobacterium bovis BCG strains Moreau and Pasteur
Description	The BCG vaccine (Bacille Calmette-Guerin), only prophylactic measure against tuberculosis (TB), was obtained in the early twentieth century by Calmette and Guérin after 231 passages of a M. bovis clinical isolate in medium containing glycerin and bovine bile. Its protective efficacy against pulmonary TB in adults varies from 0-80% and the genetic differences among vaccines strains used worldwide contribute to this variation. The Brazilian vaccine strain, BCG Moreau, is considered a primitive strain and more immunogenic, closer to the original BCG when compared to newer strains, such as BCG Pasteur (reference strain). The characterization of BCG sub-strains can contribute not only to a better understanding of the vaccine and its protective effect, but also, in elucidating how different BCG culture conditions may contribute to the impact on the host's immune response. Thus, we aimed to characterize the differences in gene expression through the intracellular proteomic profile of BCG Moreau and Pasteur strains, cultivated in Sauton or 7H9 medium, using the methodology of two-dimensional electrophoresis (2DE) and mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2025-10-23
AnnouncementXML	Submission_2025-10-23_10:17:50.731.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Paloma Corrêa
SpeciesList	scientific name: Mycobacterium tuberculosis variant bovis BCG str. Moreau; NCBI TaxID: NEWT:1195466;
ModificationList	acetylated residue; formylated residue; deamidated residue
Instrument	4700 Proteomics Analyzer

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-05 04:28:29	ID requested
⏵ 1	2025-10-23 10:17:51	announced

Dataset with its publication pending

submitter keyword: intracellular proteome, growth conditions,Mycobacterium bovis BCG Moreau

Paloma Rezende Corrêa
contact affiliation	Laboratório de Genômica Funcional e Bioinformática- IOC/FIOCRUZ - RJ/Brasil
contact email	pah.rez.correa@gmail.com
lab head
Paloma Corrêa
contact affiliation	Fiocruz
contact email	pah.rez.correa@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030179
     1. Label: PRIDE project
     2. Name: Cultivation impact on the protein expression profile of Mycobacterium bovis BCG strains Moreau and Pasteur