PXD030175

PXD030175 is an original dataset announced via ProteomeXchange.

Title	Mitogen-activated protein kinase 4 regulated metabolic networks
Description	Mitogen-activated protein kinase 4 (MPK4) was first identified as a negative regulator of systemic acquired resistance (SAR). It is also an important kinase that gets involved in other plant biological processes in plants, including cytokinesis, reproduction and photosynthesis. Arabidopsis thaliana mpk4 mutant is a dwarf and sterile. Previous ‘omics’ studies including genomics, transcriptomics and proteomics have revealed new functions of MPK4 in different biological processes. However, due to challenges in metabolomics, no study has focused on metabolomic profiles of the mpk4 mutant and what metabolites and metabolic pathways are potentially regulated by MPK4 are not known. Metabolites are crucial components of plants, which plays an and they play important roles in plant signaling, defense, and growth and development. Here we used targeted and untargeted metabolomics to profile metabolites in wild type (WT) and the mpk4 mutant where we found that in addition to jasmonic acid (JA) and salicylic acid (SA) pathways, MPK4 got involved in polyamine synthesis and photosynthesis. In addition, we also conducted label-free proteomics of the two genotypes. Integration of metabolomics and proteomics data allowed insight into the metabolomic networks that are potentially regulated by MPK4.
HostingRepository	PRIDE
AnnounceDate	2022-02-15
AnnouncementXML	Submission_2022-02-15_09:57:12.217.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chuwei Lin
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-03 09:03:31	ID requested
⏵ 1	2022-02-15 09:57:13	announced

Lin C, Lott AA, Zhu W, Dufresne CP, Chen S, Mitogen-Activated Protein Kinase 4-Regulated Metabolic Networks. Int J Mol Sci, 23(2):(2022) [pubmed]

submitter keyword: Arabidopsis thaliana, MPK4

Sixue Chen
contact affiliation	University of Florida
contact email	schen@ufl.edu
lab head
Chuwei Lin
contact affiliation	University of Florida
contact email	chuwei.lin@ufl.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030175
     1. Label: PRIDE project
     2. Name: Mitogen-activated protein kinase 4 regulated metabolic networks