<<< Full experiment listing

PXD030171

PXD030171 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleExposure to Pseudomonas aeruginosa secretome alters the proteome and secondary metabolite production of Aspergillus fumigatus.
DescriptionThe fungal pathogen Aspergillus fumigatus is frequently cultured from the sputum of cystic fibrosis (CF) patients along with the bacterium, Pseudomonas aeruginosa. A. fumigatus secretes a range of secondary metabolites, and one of these, gliotoxin, has inhibitory effects on the host immune response. In this study, the effect of P. aeruginosa culture filtrate (CuF) on fungal growth and gliotoxin production was investigated. Exposure of A. fumigatus hyphae to P. aeruginosa cells induced increased production of gliotoxin and a decrease in fungal growth. In contrast exposure of A. fumigatus hyphae to P. aeruginosa CuF lead to increased growth and decreased gliotoxin production. Quantitative proteomic analysis was employed to characterize the proteomic response of A. fumigatus upon exposure to P. aeruginosa CuF. Changes in the profile of proteins involved with secondary metabolite biosynthesis (gliotoxin, fumagillin, pseurotin A), and changes to the abundance of proteins involved in oxidative stress (e.g. formate dehydrogenase) and detoxification (e.g. thioredoxin reductase) were observed, suggesting that the bacterial secretome has a profound effect on the fungal proteome. Alterations in the abundance of proteins involved in detoxification and oxidative stress, highlight the ability of A. fumigatus to differentially regulate protein synthesis in response to environmental stresses imposed by competitors such as P. aeruginosa. Such responses may ultimately have serious detrimental effects on the host.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_07:51:33.843.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJames Carolan
SpeciesList scientific name: Neosartorya fumigata (Aspergillus fumigatus); NCBI TaxID: 746128;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-12-03 06:57:02ID requested
12023-03-10 13:14:30announced
22023-11-14 07:51:40announced2023-11-14: Updated project metadata.
Publication List
Margalit A, Sheehan D, Carolan JC, Kavanagh K, . Microbiology (Reading), 168(3):(2022) [pubmed]
Keyword List
submitter keyword: culture filtrate, LFQ proteomics,Aspergillus fumigatus, Pseudomonas aeruginosa
Contact List
Jim Carolan
contact affiliationApplied Proteomics Lab, Department of Biology, Maynooth University, Ireland.
contact emailjames.carolan@nuim.ie
lab head
James Carolan
contact affiliationBiology
contact emailjames.carolan@nuim.ie
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD030171
PRIDE project URI
Repository Record List
[ + ]