PXD030166

PXD030166 is an original dataset announced via ProteomeXchange.

Title	Aggregation under genotoxic conditions in HEK293T and U2OS cells
Description	This dataset consists of 2 independent experiments. The first research aim was to identify the proteins that aggregate in HEK293T cells treated with the chemotherapeutic agent camptothecin (CPT), the second to identify the proteins that aggregate in U2OS cells after a loss of the checkpoint kinase ATM. For the first experiment, cells were treated for 24 hours with vehicle (DMSO) or CPT, left to recover for 48 hours, and harvested. For the second experiment, wild-type and ATM KO cells were grown for 72 hours and harvested. For both experiments, aggregated proteins were isolated by a differential detergent fractionation protocol, which ultimately resulted in a 1% SDS insoluble protein fraction. These proteins were solubilized in 8M urea.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:00:52.209.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luciano Di Stefano
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-03 06:38:47	ID requested
1	2022-02-01 08:12:27	announced
2	2022-03-15 04:55:01	announced	2022-03-15: Updated project metadata.
3	2022-04-07 09:16:26	announced	2022-04-07: Updated project metadata.
4	2022-04-07 15:18:49	announced	2022-04-07: Updated project metadata.
⏵ 5	2023-11-14 09:00:58	announced	2023-11-14: Updated project metadata.

Huiting W, Dekker SL, van der Lienden JCJ, Mergener R, Musskopf MK, Furtado GV, Gerrits E, Coit D, Oghbaie M, Di Stefano LH, Schepers H, van Waarde-Verhagen MAWH, Couzijn S, Barazzuol L, LaCava J, Kampinga HH, Bergink S, Targeting DNA topoisomerases or checkpoint kinases results in an overload of chaperone systems, triggering aggregation of a metastable subproteome. Elife, 11():(2022) [pubmed]

submitter keyword: Human, insoluble, genotoxic, CPT, U2OS, camptothecin, aggregation

John LaCava
contact affiliation	Laboratory of Macromolecules and Interactomes,ERIBA-UMCG, Groningen, The Netherlands
contact email	j.p.lacava@umcg.nl
lab head
Luciano Di Stefano
contact affiliation	ERIBA-UMCG
contact email	l.h.di.stefano@umcg.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD030166

PRIDE project URI

• PRIDE
  1. PXD030166
     1. Label: PRIDE project
     2. Name: Aggregation under genotoxic conditions in HEK293T and U2OS cells