PXD030133

PXD030133 is an original dataset announced via ProteomeXchange.

Title	Carrot (Daucus carota L.) seed germination was promoted by hydro-electro hybrid priming through regulating the accumulation of proteins involved in carbohydrate and protein metabolism
Description	Asynchronized and nonuniform seed germination is causing obstacles to the large-scale cultivation of carrot (Daucus carota L.). In the present study, the combination of high voltage electrostatic field treatment (EF) with hydropriming (HYD), namely hydro-electro hybrid priming (HEHP), significantly improved all germination indicators of carrot seeds, and the promoting effect was superior to that of the HYD treatment. A TMT-based proteomic analysis was conducted on the carrot seeds, and the maximum number of differentially abundant proteins (DAPs) appeared between CK and HEHP. KEGG analysis revealed that the upregulated DAPs were mainly enriched in the pathways related to protein synthesis and degradation such as “ribosome” and “proteasome”, while the downregulated DAPs were mainly enriched in photosynthesis-related pathways. Furthermore, the maximum DAPs were annotated in carbohydrate metabolism. Some proteins identified as key enzymes of the glyoxylate cycle, the tricarboxylate cycle, glycolysis and the pentose phosphate pathway showed enhanced abundance in priming treatments. The activities of several key enzymes involved in carbohydrate metabolism were also enhanced by the priming treatments, especially the HEHP treatment. Real-time quantitative PCR (qRT-PCR) analysis revealed that the effect of priming is mainly reflected before sowing. In conclusion, the optimal effect of HEHP is to regulate the synthesis and degradation of proteins in seeds to meet the requirements of germination and initiate the utilization of seed storage reserves and respiratory metabolism. The present work expanded the understanding of the response mechanism of carrot seed germination to priming and the biological effects of high voltage electrostatic field.
HostingRepository	PRIDE
AnnounceDate	2022-05-27
AnnouncementXML	Submission_2022-05-27_02:51:43.655.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shuo Zhao
SpeciesList	scientific name: Daucus carota subsp. sativus; NCBI TaxID: 79200;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF; LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-02 08:03:18	ID requested
⏵ 1	2022-05-27 02:51:44	announced

Zhao S, Zou H, Jia Y, Pan X, Huang D, L.) Seed Germination Was Promoted by Hydro-Electro Hybrid Priming Through Regulating the Accumulation of Proteins Involved in Carbohydrate and Protein Metabolism. Front Plant Sci, 13():824439(2022) [pubmed]

submitter keyword: hydro-electro hybrid priming, carrot, seed germination, TMT-based proteomic analysis, protein synthesis and degradation, carbohydrate metabolism

Shuo Zhao
contact affiliation	School of Agriculture & Biology, Shanghai Jiao Tong University
contact email	zhaoshuo@sjtu.edu.cn
lab head
Shuo Zhao
contact affiliation	Shanghai Jiao Tong University
contact email	zhaoshuo@sjtu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030133
     1. Label: PRIDE project
     2. Name: Carrot (Daucus carota L.) seed germination was promoted by hydro-electro hybrid priming through regulating the accumulation of proteins involved in carbohydrate and protein metabolism