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PXD030133

PXD030133 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCarrot (Daucus carota L.) seed germination was promoted by hydro-electro hybrid priming through regulating the accumulation of proteins involved in carbohydrate and protein metabolism
DescriptionAsynchronized and nonuniform seed germination is causing obstacles to the large-scale cultivation of carrot (Daucus carota L.). In the present study, the combination of high voltage electrostatic field treatment (EF) with hydropriming (HYD), namely hydro-electro hybrid priming (HEHP), significantly improved all germination indicators of carrot seeds, and the promoting effect was superior to that of the HYD treatment. A TMT-based proteomic analysis was conducted on the carrot seeds, and the maximum number of differentially abundant proteins (DAPs) appeared between CK and HEHP. KEGG analysis revealed that the upregulated DAPs were mainly enriched in the pathways related to protein synthesis and degradation such as “ribosome” and “proteasome”, while the downregulated DAPs were mainly enriched in photosynthesis-related pathways. Furthermore, the maximum DAPs were annotated in carbohydrate metabolism. Some proteins identified as key enzymes of the glyoxylate cycle, the tricarboxylate cycle, glycolysis and the pentose phosphate pathway showed enhanced abundance in priming treatments. The activities of several key enzymes involved in carbohydrate metabolism were also enhanced by the priming treatments, especially the HEHP treatment. Real-time quantitative PCR (qRT-PCR) analysis revealed that the effect of priming is mainly reflected before sowing. In conclusion, the optimal effect of HEHP is to regulate the synthesis and degradation of proteins in seeds to meet the requirements of germination and initiate the utilization of seed storage reserves and respiratory metabolism. The present work expanded the understanding of the response mechanism of carrot seed germination to priming and the biological effects of high voltage electrostatic field.
HostingRepositoryPRIDE
AnnounceDate2022-05-27
AnnouncementXMLSubmission_2022-05-27_02:51:43.655.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterShuo Zhao
SpeciesList scientific name: Daucus carota subsp. sativus; NCBI TaxID: 79200;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF; LTQ
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-12-02 08:03:18ID requested
12022-05-27 02:51:44announced
Publication List
Zhao S, Zou H, Jia Y, Pan X, Huang D, L.) Seed Germination Was Promoted by Hydro-Electro Hybrid Priming Through Regulating the Accumulation of Proteins Involved in Carbohydrate and Protein Metabolism. Front Plant Sci, 13():824439(2022) [pubmed]
Keyword List
submitter keyword: hydro-electro hybrid priming, carrot, seed germination, TMT-based proteomic analysis, protein synthesis and degradation, carbohydrate metabolism
Contact List
Shuo Zhao
contact affiliationSchool of Agriculture & Biology, Shanghai Jiao Tong University
contact emailzhaoshuo@sjtu.edu.cn
lab head
Shuo Zhao
contact affiliationShanghai Jiao Tong University
contact emailzhaoshuo@sjtu.edu.cn
dataset submitter
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Dataset FTP location
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