PXD030125

PXD030125 is an original dataset announced via ProteomeXchange.

Title	Campylobacter jejuni virulence factors identified by modulating their sCampylobacter jejuni virulence factors identified by modulating their synthesis on ribosomes with altered rRNA methylationynthesis on ribosomes with altered rRNA methylation
Description	Campylobacter jejuni is a major cause of food poisoning worldwide, and remains the main infective agent in gastroenteritis and related intestinal disorders in Europe and the USA. As with all bacterial infections, the stages of adhesion to host tissue, survival in the host and eliciting disease all require the synthesis of proteinaceous virulence factors on the ribosomes of the pathogen. Here, we describe how C. jejuni virulence is attenuated by altering the methylation of its ribosomes to disrupt the composition of its proteome, and how this in turn provides a means of identifying factors that are essential for infection and pathogenesis. Specifically, inactivation of the C. jejuni Cj0588/TlyA methyltransferase prevents methylation of nucleotide C1920 in the 23S rRNA of its ribosomes and reduces the pathogen’s ability to form biofilms, to attach, invade and survive in host cells, and to provoke the innate immune response. Mass spectrometric analyses of C. jejuni TlyA-minus strains revealed an array of subtle changes in the proteome composition. These included reduced amounts of the cytolethal distending toxin (CdtC) and the MlaEFD proteins connected with outer membrane vesicle (OMV) production. Inactivation of the cdtC and mlaEFD genes confirms the importance of the toxin and OMVs in establishing infection. Collectively, the data identify a collection of genes required for the onset of human campylobacteriosis, and serve as a proof of principle for use of this approach in detecting proteins involved in bacterial pathogenesis.
HostingRepository	PRIDE
AnnounceDate	2022-01-26
AnnouncementXML	Submission_2022-02-09_08:45:54.664.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Agnieszka Sałamaszyńska-Guz
SpeciesList	scientific name: Campylobacter jejuni subsp. jejuni; NCBI TaxID: 32022;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-02 04:21:43	ID requested
1	2022-01-26 12:28:57	announced
⏵ 2	2022-02-09 08:45:55	announced	2022-02-09: Updated publication reference for PubMed record(s): 35096652.

Sa, ł, amaszy, ń, ska-Guz A, Rasmussen PK, Murawska M, Douthwaite S, Virulence Factors Identified by Modulating Their Synthesis on Ribosomes With Altered rRNA Methylation. Front Cell Infect Microbiol, 11():803730(2021) [pubmed]

submitter keyword: Campylobacter

Agnieszka Sałamaszyńska-Guz
contact affiliation	Department of Pre-Clinical Sciences, Institute of Veterinary Medicine, Warsaw University of Live Sciences – SGGW Poland
contact email	agnieszka_salamaszynska_guz@sggw.edu.pl
lab head
Agnieszka Sałamaszyńska-Guz
contact affiliation	Institute of Veterinary Medicine, Warsaw University of Live Sciences – SGGW
contact email	agnieszka_salamaszynska_guz@sggw.edu.pl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030125
     1. Label: PRIDE project
     2. Name: Campylobacter jejuni virulence factors identified by modulating their sCampylobacter jejuni virulence factors identified by modulating their synthesis on ribosomes with altered rRNA methylationynthesis on ribosomes with altered rRNA methylation