PXD030122

PXD030122 is an original dataset announced via ProteomeXchange.

Title	AP-MS of MAGU-2 in the nematode C. elegans
Description	The Crumbs complex is a regulator of epithelial polarity and is important for the formation of the apical domain. The Crumbs complex consists of the Crumbs protein, Pals1, and PATJ. In C. elegans, we identify MAGU-2 as an ortholog of Pals1. We find that MAGU-2 localizes apically in epithelial cells and its localization is dependent on Crumbs. AP-MS was performed on animals endogenously expressing MAGU-2::GFP and four control strains to identify interactors of MAGU-2. From the data, we identify MPZ-1 as the top hit and identify it as the C. elegans ortholog of PATJ.
HostingRepository	PRIDE
AnnounceDate	2022-05-19
AnnouncementXML	Submission_2022-05-19_14:53:36.177.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jason Kroll
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-02 04:01:24	ID requested
⏵ 1	2022-05-19 14:53:36	announced

Castiglioni VG, Ramalho JJ, Kroll JR, Stucchi R, van Beuzekom H, Schmidt R, Altelaar M, Boxem M, Identification and characterization of Crumbs polarity complex proteins in Caenorhabditis elegans. J Biol Chem, 298(4):101786(2022) [pubmed]

submitter keyword: C. elegans, MAGU-2, Pals1, Crumbs

Mike Boxem
contact affiliation	Developmental Biology, Institute of Biodynamics and Biocomplexity, Department of Biology, Faculty of Science, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands
contact email	m.boxem@uu.nl
lab head
Jason Kroll
contact affiliation	Developmental Biology Utrecht University
contact email	j.r.kroll@uu.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD030122

PRIDE project URI

• PRIDE
  1. PXD030122
     1. Label: PRIDE project
     2. Name: AP-MS of MAGU-2 in the nematode C. elegans