PXD030113

PXD030113 is an original dataset announced via ProteomeXchange.

Title	Protein interaction network analysis reveals growth conditions-specific crosstalk between chromosomal DNA replication and other cellular processes in E. coli
Description	E. coli and many other bacterial species can alternate their cell cycle according to nutrient availability. As a result, they grow and divide very fast under optimal conditions or slow down the cell cycle when conditions deteriorate. This adaptability is underlined by mechanisms coordinating cell growth with duplication of genetic material and cell division. Several mechanisms regulating DNA replication process in E. coli have been described so far with biochemical details, nevertheless we still don’t fully understand the source of remarkable precision that bacterial cells coordinate their growth and chromosome replication with. To shed light on regulation of E. coli DNA replication at systemic level, we used affinity purification coupled with mass spectrometry (AP-MS) to characterize protein-protein interactions (PPIs) formed by key E. coli replication proteins, under disparate bacterial growth conditions and phases. We present the resulting dynamic replication protein interaction network (PIN) and highlight links between DNA replication and several cellular processes, like outer membrane synthesis, RNA degradation and modification or starvation response.
HostingRepository	PRIDE
AnnounceDate	2022-03-03
AnnouncementXML	Submission_2022-03-03_03:21:34.877.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dominik Cysewski
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-02 03:24:39	ID requested
⏵ 1	2022-03-03 03:21:35	announced

Dataset with its publication pending

submitter keyword: chromosmal DNA, e.coli, ap-ms

Monika Glinkowska
contact affiliation	Department of Bacterial Molecular Genetics University of Gdansk
contact email	monika.glinkowska@ug.edu.pl
lab head
Dominik Cysewski
contact affiliation	Institute of Biochemistry and Biophysics Polish Academy of Sciences
contact email	dominikcysewski@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD030113

PRIDE project URI

• PRIDE
  1. PXD030113
     1. Label: PRIDE project
     2. Name: Protein interaction network analysis reveals growth conditions-specific crosstalk between chromosomal DNA replication and other cellular processes in E. coli