PXD030113 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Protein interaction network analysis reveals growth conditions-specific crosstalk between chromosomal DNA replication and other cellular processes in E. coli |
Description | E. coli and many other bacterial species can alternate their cell cycle according to nutrient availability. As a result, they grow and divide very fast under optimal conditions or slow down the cell cycle when conditions deteriorate. This adaptability is underlined by mechanisms coordinating cell growth with duplication of genetic material and cell division. Several mechanisms regulating DNA replication process in E. coli have been described so far with biochemical details, nevertheless we still don’t fully understand the source of remarkable precision that bacterial cells coordinate their growth and chromosome replication with. To shed light on regulation of E. coli DNA replication at systemic level, we used affinity purification coupled with mass spectrometry (AP-MS) to characterize protein-protein interactions (PPIs) formed by key E. coli replication proteins, under disparate bacterial growth conditions and phases. We present the resulting dynamic replication protein interaction network (PIN) and highlight links between DNA replication and several cellular processes, like outer membrane synthesis, RNA degradation and modification or starvation response. |
HostingRepository | PRIDE |
AnnounceDate | 2022-03-03 |
AnnouncementXML | Submission_2022-03-03_03:21:34.877.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Dominik Cysewski |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; |
ModificationList | acetylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-12-02 03:24:39 | ID requested | |
⏵ 1 | 2022-03-03 03:21:35 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: chromosmal DNA, e.coli, ap-ms |
Contact List
Monika Glinkowska |
contact affiliation | Department of Bacterial Molecular Genetics University of Gdansk |
contact email | monika.glinkowska@ug.edu.pl |
lab head | |
Dominik Cysewski |
contact affiliation | Institute of Biochemistry and Biophysics Polish Academy of Sciences |
contact email | dominikcysewski@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD030113
- Label: PRIDE project
- Name: Protein interaction network analysis reveals growth conditions-specific crosstalk between chromosomal DNA replication and other cellular processes in E. coli