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PXD030113

PXD030113 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProtein interaction network analysis reveals growth conditions-specific crosstalk between chromosomal DNA replication and other cellular processes in E. coli
DescriptionE. coli and many other bacterial species can alternate their cell cycle according to nutrient availability. As a result, they grow and divide very fast under optimal conditions or slow down the cell cycle when conditions deteriorate. This adaptability is underlined by mechanisms coordinating cell growth with duplication of genetic material and cell division. Several mechanisms regulating DNA replication process in E. coli have been described so far with biochemical details, nevertheless we still don’t fully understand the source of remarkable precision that bacterial cells coordinate their growth and chromosome replication with. To shed light on regulation of E. coli DNA replication at systemic level, we used affinity purification coupled with mass spectrometry (AP-MS) to characterize protein-protein interactions (PPIs) formed by key E. coli replication proteins, under disparate bacterial growth conditions and phases. We present the resulting dynamic replication protein interaction network (PIN) and highlight links between DNA replication and several cellular processes, like outer membrane synthesis, RNA degradation and modification or starvation response.
HostingRepositoryPRIDE
AnnounceDate2022-03-03
AnnouncementXMLSubmission_2022-03-03_03:21:34.877.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDominik Cysewski
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationListacetylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-12-02 03:24:39ID requested
12022-03-03 03:21:35announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: chromosmal DNA, e.coli, ap-ms
Contact List
Monika Glinkowska
contact affiliationDepartment of Bacterial Molecular Genetics University of Gdansk
contact emailmonika.glinkowska@ug.edu.pl
lab head
Dominik Cysewski
contact affiliationInstitute of Biochemistry and Biophysics Polish Academy of Sciences
contact emaildominikcysewski@gmail.com
dataset submitter
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Dataset FTP location
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