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PXD030106

PXD030106 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRbp95 binds 25S rRNA helix H95 and cooperates with the Npa1 complex during early pre-60S particle maturation
DescriptionEukaryotic ribosome synthesis involves more than 200 assembly factors, which promote ribosomal RNA (rRNA) processing, modification and folding, and assembly of ribosomal proteins. The formation and maturation of the earliest pre-60S particles requires structural remodeling by the Npa1 complex, but is otherwise still poorly understood. Here we introduce Rbp95, a constituent of early pre-60S particles, as a novel ribosome assembly factor that likely already binds before the 90S to pre-60S transition. We show that Rbp95 is both genetically and physically linked to most Npa1 complex members, and to ribosomal protein Rpl3. Notably, the RNA-binding protein Rbp95 associates with helix H95 in the 3’ region of the 25S rRNA, in close proximity to the binding sites of Npa1 and Rpl3. Additionally, Rbp95 interacts with several snoRNAs. In the absence of Rbp95, proteins binding in proximity to helix H95 are less efficiently recruited to early pre-60S particles. Moreover, combined mutation of Rbp95 and Npa1 complex members leads to a delay in the formation of early pre-60S particles. We propose that Rbp95 acts together with the Npa1 complex in the 90S to pre-60S transition and in promoting pre-rRNA folding events within pre-60S particles that facilitate the recruitment of subsequent assembly factors.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:14:35.269.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBarbara Darnhofer
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos; maXis
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-12-02 02:54:25ID requested
12022-10-13 09:02:15announced
22023-11-14 08:14:36announced2023-11-14: Updated project metadata.
Publication List
Bhutada P, Favre S, Jaafar M, Hafner J, Liesinger L, Unterweger S, Bischof K, Darnhofer B, Siva Sankar D, Rechberger G, Abou Merhi R, Lebaron S, Birner-Gruenberger R, Kressler D, Henras AK, Pertschy B, Rbp95 binds to 25S rRNA helix H95 and cooperates with the Npa1 complex during early pre-60S particle maturation. Nucleic Acids Res, 50(17):10053-10077(2022) [pubmed]
Keyword List
submitter keyword: ribosome assembly factor, yeast, S. cerevisiae, Tandem-Affinity Purification (TAP), Npa1, pre-60S particles,Rbp95
Contact List
Ruth Birner-Gruenberger
contact affiliationResearch and Diagnostic Institute of Pathology, Medical University of Graz, Stiftingtalstrasse 6, 8010 Graz, Austria; BioTechMed-Graz, Graz, Austria; Institute of Chemical Technologies and Analytics, Technische Universität Wien, Getreidemarkt 9/164, 1060 Vienna, Austria;
contact emailruth.birner-gruenberger@tuwien.ac.at
lab head
Barbara Darnhofer
contact affiliationOmics Center Graz
contact emailbarbara.darnhofer@klinikum-graz.at
dataset submitter
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