PXD030105

PXD030105 is an original dataset announced via ProteomeXchange.

Title	Proteomic consequences of TDA1 deficiency in Saccharomyces cerevisiae — Protein kinase Tda1 is essential for Hxk1 and Hxk2 serine 15 phosphorylation
Description	Hexokinase 2 (Hxk2) of Saccharomyces cerevisiae is a dual function hexokinase, acting as a glycolytic enzyme and being involved in the transcriptional regulation of glucose-repressible genes. Relief from glucose repression is accompanied by phosphorylation of Hxk2 at serine 15, which has been attributed to the protein kinase Tda1. To explore the role of Tda1 beyond Hxk2 phosphorylation the proteomic consequences of TDA1 deficiency were investigated by difference gel electrophoresis (2D-DIGE) comparing a wild type and a Δtda1 deletion mutant. Both were grown at 2 % and 0.1 % (w/v) glucose to additionally regard glucose repression/derepression. A total of eight spots exhibiting a minimum 2-fold enhanced or reduced fluorescence upon TDA1 deficiency was detected and identified by mass spectrometry. These include – besides the expected Hxk2 – two proteoforms of hexokinase 1 (Hxk1). Targeted proteomics analyses in conjunction with 2D-DIGE demonstrated that TDA1 is indispensable for Hxk2 and Hxk1 phosphorylation at serine 15. 35 glucose-dependent proteins were identified. To improve spot quantification, a simple method approximating spots as rotationally symmetric solids is presented along with new data on the quantities of Hxk1 and Hxk2 and their serine 15 phosphorylated forms at high and low glucose growth conditions. The Δtda1 deletion mutant exhibited no altered growth comparing high or low glucose conditions or on alternative carbon sources. Also, invertase activity, serving as a reporter for glucose derepression, was not significantly altered. Instead, an involvement of Tda1 in oxidative stress response is suggested.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:24:28.336.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marc Gentzel
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	L-methionine sulfoxide
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-02 02:51:29	ID requested
1	2023-02-06 09:51:04	announced
⏵ 2	2023-11-14 08:24:36	announced	2023-11-14: Updated project metadata.

M, ü, ller H, Lesur A, Dittmar G, Gentzel M, Kettner K, Proteomic consequences of TDA1 deficiency in Saccharomyces cerevisiae: Protein kinase Tda1 is essential for Hxk1 and Hxk2 serine 15 phosphorylation. Sci Rep, 12(1):18084(2022) [pubmed]

submitter keyword: Yeast, In-Gel Digestion, Saccharomyces cerevisiae, Hexokinase,2D Gel elctrophoresis, LC-MS/MS

Dr. Karina Kettner
contact affiliation	Technische Universität Dresden, Medizinische Fakultät Carl Gustav Carus, Institute of Physiological Chemistry, 01307 Dresden, Germany
contact email	karina.kettner@tu-dresden.de
lab head
Marc Gentzel
contact affiliation	Molecular Analysis - Mass Spectrometry, Center for Molecular and Cellular Bioengineering (CMCB), TU Dresden
contact email	marc.gentzel@tu-dresden.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030105
     1. Label: PRIDE project
     2. Name: Proteomic consequences of TDA1 deficiency in Saccharomyces cerevisiae — Protein kinase Tda1 is essential for Hxk1 and Hxk2 serine 15 phosphorylation