PXD030103

PXD030103 is an original dataset announced via ProteomeXchange.

Title	Proteomic Profiling and Functional Analysis of B Cell-Derived Exosomes upon Pneumocystis Infection
Description	Pneumocystis is a life-threatening fungal pathogen frequently causing fatal pneumonia (PCP) in immunocompromised individuals with high mortality. Recently, B cells have been reported to play a crucial role in the pathogenesis of PCP through producing antibodies and activating CD4+ T cell response. Exosomes are nano-scale small extracellular vesicles (EVs) abundant of protein cargo and can mediate immune response during infectious disease. Here, using quantitative proteomic analysis coupled with bioinformatic analysis, we attempted to characterize exosomes derived from B lymphocytes in response to PCP. Also, the effects of B-cell exosomes on CD4+ T cell response and phagocytic function of macrophages were clarified. Briefly, 1701 proteins were identified from B-cell exosomes and the majority of them were reported in Vesiclepedia. 51 differentially expressed proteins of B-cell exosomes were found in response to PCP. They were mainly associated with immune response and transcription regulation. Particularly, a variety of histone components were enriched in B cell-derived exosomes when infected with Pneumocystis. Moreover, functional study revealed the pro-inflammatory profile of B-cell exosomes on CD4+ T cell response in PCP. Taken together, our results suggest the involvement of exosomes derived from B cells in cell-to-cell communication, providing new information on the function of B cells in response to PCP.
HostingRepository	PRIDE
AnnounceDate	2022-04-26
AnnouncementXML	Submission_2022-04-26_01:09:00.550.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhao-Hui Tong
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-02 02:37:27	ID requested
⏵ 1	2022-04-26 01:09:12	announced

Ma D, Zhang QY, Rong HM, Zhai K, Tong ZH, Infection. J Immunol Res, 2022():5187166(2022) [pubmed]

submitter keyword: Pneumocystis pneumonia, B lymphocytes, exosomes, mass spectrometry

Zhaohui Tong
contact affiliation	Department of Respiratory and Critical Care Medicine, Beijing Institute of Respiratory Medicine and Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China
contact email	tongzhaohuicy@sina.com
lab head
Zhao-Hui Tong
contact affiliation	Department of Respiratory and Critical Care Medicine, Beijing Institute of Respiratory Medicine and Beijing Chao-Yang Hospital, Capital Medical University
contact email	tongzhaohuicy@sina.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD030103

PRIDE project URI

• PRIDE
  1. PXD030103
     1. Label: PRIDE project
     2. Name: Proteomic Profiling and Functional Analysis of B Cell-Derived Exosomes upon Pneumocystis Infection