PXD030095 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Multi-facetted proteomics of mitochondrial TMBIM5 deficient cell lines: Regulation of mitochondrial proteostasis by the proton gradient |
Description | Mitochondria adapt to different energetic demands reshaping their proteome. Mitochondrial proteases are emerging as key regulators of these adaptive processes. Here, we use a multi-proteomic approach to demonstrate regulation of the m-AAA protease AFG3L2 by the mitochondrial proton gradient, coupling mitochondrial protein turnover to the energetic status of mitochondria. We identify TMBIM5 (previously also known as GHITM or MICS1) as a Ca2+/H+ exchanger in the mitochondrial inner membrane, which binds to and inhibits the m-AAA protease. TMBIM5 ensures cell survival and respiration, allowing Ca2+ efflux from mitochondria and limiting mitochondrial hyperpolarization. Persistent hyperpolarization, however, triggers degradation of TMBIM5 and activation of the m-AAA protease. The m-AAA protease broadly remodels the mitochondrial proteome and mediates the proteolytic breakdown of respiratory complex I to confine ROS production and oxidative damage in hyperpolarized mitochondria. TMBIM5 thus integrates mitochondrial Ca2+ signaling and the energetic status of mitochondria with protein turnover rates to reshape the mitochondrial proteome and adjust the cellular metabolism. |
HostingRepository | PRIDE |
AnnounceDate | 2022-07-26 |
AnnouncementXML | Submission_2022-07-26_14:52:05.178.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Hendrik Nolte |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF; Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-12-02 02:12:35 | ID requested | |
⏵ 1 | 2022-07-26 14:52:05 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: TMBIM5, AFG3L2, Mitochondria, HeLa, HEK, SILAC, Cyclohexamide, GHITM |
Contact List
Thomas Langer |
contact affiliation | Max-Planck-Institute for Biology of Ageing Department of Mitochondrial Proteostasis Joseph-Stelzmann-Str. 9b 50931 Cologne |
contact email | tlanger@age.mpg.de |
lab head | |
Hendrik Nolte |
contact affiliation | Max Planck Institute for Biology of Ageing, 50931 Cologne, Germany |
contact email | h.nolte@age.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/07/PXD030095 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD030095
- Label: PRIDE project
- Name: Multi-facetted proteomics of mitochondrial TMBIM5 deficient cell lines: Regulation of mitochondrial proteostasis by the proton gradient