PXD030078

PXD030078 is an original dataset announced via ProteomeXchange.

Title	An integrative multi-omics approach to characterize prebiotic inulin effects on Faecalibacterium prausnitzii
Description	Faecalibacterium prausnitzii, a major commensal bacterium in the human gut, is well known for its anti-inflammatory effects, which improve host intestinal health. Although several studies have reported that inulin, a well-known prebiotic, increases the abundance of F. prausnitzii in the intestine, the mechanism underlying this effect remains unclear. In this study, we applied liquid chromatography tandem mass spectrometry (LC-MS/MS)-based multi-omics approaches to identify biological and enzymatic mechanisms of F. prausnitzii involved in the selective digestion of inulin. An LC-MS/MS-based intracellular proteomic and metabolic profiling was performed to determine the quantitative changes in specific proteins and metabolites of F. prausnitzii when grown on inulin. Interestingly, proteomic analysis revealed that the putative proteins involved in inulin-type fructan utilization by F. prausnitzii, particularly b-fructosidase and amylosucrase were upregulated in the presence of inulin. To investigate the function of these proteins, we overexpressed bfrA and ams, genes encoding beta-fructosidase and amylosucrase, respectively, in Escherichia coli, and observed their ability to degrade fructan. In addition, the enzyme activity assay demonstrated that intracellular fructan hydrolases degrade the inulin-type fructans taken up by fructan ATP-binding cassette transporters. Furthermore, we showed that the fructose uptake activity of F. prausnitzii was enhanced by the fructose phosphotransferase system transporter when inulin was used as a carbon source.
HostingRepository	PRIDE
AnnounceDate	2022-05-25
AnnouncementXML	Submission_2022-05-25_09:13:01.897.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sung-Hyun Jo
SpeciesList	scientific name: Faecalibacterium prausnitzii A2-165; NCBI TaxID: 411483;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-30 15:13:41	ID requested
⏵ 1	2022-05-25 09:13:02	announced

Park JH, Song WS, Lee J, Jo SH, Lee JS, Jeon HJ, Kwon JE, Kim YR, Baek JH, Kim MG, Yang YH, Kim BG, Kim YG, . Front Bioeng Biotechnol, 10():825399(2022) [pubmed]

submitter keyword: Faecalibacterium prausnitzii, Inulin, Prebiotics, LC-MS/MS, beta-fructosidase, Amylosucrase, ABC transporter, PTS transporter

Yon-Gon Kim
contact affiliation	Department of Chemical Engineering, Laboratory of Cellular and Biomolecular Engineering, Soongsil University, Republic of Korea
contact email	ygkim@ssu.ac.kr
lab head
Sung-Hyun Jo
contact affiliation	Soongsil university
contact email	sh20511@soongsil.ac.kr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030078
     1. Label: PRIDE project
     2. Name: An integrative multi-omics approach to characterize prebiotic inulin effects on Faecalibacterium prausnitzii