PXD030071

PXD030071 is an original dataset announced via ProteomeXchange.

Title	S-nitrosation of Arabidopsis thaliana Protein Tyrosine Phosphatase 1 (AtPTP1) prevents its irreversible oxidation by hydrogen peroxide
Description	Tyrosine-specific protein tyrosine phosphatases (Tyr-specific PTPases) are key signalling enzymes catalyzing the removal of the phosphate group from phosphorylated tyrosine residues on target proteins. This post-translational modification notably allows the regulation of mitogen-activated protein kinase (MAPK) cascades during defense reactions. Arabidopsis thaliana protein tyrosine phosphatase 1 (AtPTP1), the only Tyr-specific PTPase present in this plant, acts as a repressor of H2O2 production and regulates the activity of MPK3/MPK6 MAPKs by direct dephosphorylation. Here, we report that recombinant His-AtPTP1 protein activity is directly inhibited by H2O2 and nitric oxide (NO) exogenous treatments. The effects of NO are exerted by S-nitrosation, i.e. the formation of a covalent bond between NO and a reduced cysteine residue. This post-translational modification targets the catalytic cysteine C265 and could protect the AtPTP1 protein from its irreversible oxidation by H2O2. This mechanism of protection could be a conserved mechanism in all plant PTPases
HostingRepository	PRIDE
AnnounceDate	2022-05-27
AnnouncementXML	Submission_2022-05-27_02:51:28.967.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Carole Pichereaux
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	nitrosylated residue; methylthiolated residue; biotinylated residue; iodoacetamide derivatized residue; L-cysteine glutathione disulfide
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-30 13:11:52	ID requested
⏵ 1	2022-05-27 02:51:29	announced

Nicolas-Franc, è, s V, Rossi J, Rosnoblet C, Pichereaux C, Hichami S, Astier J, Klinguer A, Wendehenne D, Besson-Bard A, Protein Tyrosine Phosphatase 1 Prevents Its Irreversible Oxidation by Hydrogen Peroxide. Front Plant Sci, 13():807249(2022) [pubmed]

submitter keyword: Arabidopsis thaliana, protein tyrosine phosphatase 1, Nitric Oxide, S-nitrosation, H2O2, Oxidation, post-translational modifications, Mitogen-Activated Protein Kinases

Besson-Bard Angelique
contact affiliation	UMR 1347 Agroécologie Agrosup Dijon / INRA / Université de Bourgogne / Dijon / France
contact email	angelique.besson-bard@inrae.fr
lab head
Carole Pichereaux
contact affiliation	CNRS
contact email	Carole.pichereaux@ipbs.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD030071

PRIDE project URI

• PRIDE
  1. PXD030071
     1. Label: PRIDE project
     2. Name: S-nitrosation of Arabidopsis thaliana Protein Tyrosine Phosphatase 1 (AtPTP1) prevents its irreversible oxidation by hydrogen peroxide