PXD030069

PXD030069 is an original dataset announced via ProteomeXchange.

Title	A census of Hsp70-mediated proteome solubility changes upon recovery from severe heat stress
Description	Eukaryotic cells respond to heat shock through several regulatory processes including upregulation of stress responsive chaperones and reversible shutdown cellular activities through formation of protein assemblies. However, the underlying regulatory mechanisms of the recovery of these heat-induced protein assemblies remain largely elusive. Here, we measured the proteome abundance and solubility changes during recovery from severe heat shock in the mouse Neuro2a cell line. We found that prefoldins and translation machinery are rapidly down-regulated as the first step in the heat shock response. Analysis of proteome solubility reveals a rapid mobilization of protein quality control machineries, changes in cellular energy metabolism, changes in translational activity and nucleocytoplasmic transport are fundamental to the early stress responses, while the longer term adaptation to stress involves renewal of core cellular components. Hsp70 inhibition negatively affects the ribosomal machinery and delays the solubility recovery of many nuclear proteins.
HostingRepository	PRIDE
AnnounceDate	2022-03-08
AnnouncementXML	Submission_2022-03-07_19:21:42.802.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD030069
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Shuai Nie
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-30 13:09:52	ID requested
⏵ 1	2022-03-07 19:21:43	announced

Dataset with its publication pending

submitter keyword: heat shock, chaperone, recovery, protein solubility, heat shock protein 70, disaggregation

Danny M. Hatters
contact affiliation	Department of Biochemistry and Pharmacology, The University of Melbourne, VIC 3010. Australia; Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, VIC 3010. Australia
contact email	dhatters@unimelb.edu
lab head
Shuai Nie
contact affiliation	The University of Melbourne
contact email	shuai.nie@unimelb.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030069
     1. Label: PRIDE project
     2. Name: A census of Hsp70-mediated proteome solubility changes upon recovery from severe heat stress