PXD030063

PXD030063 is an original dataset announced via ProteomeXchange.

Title	Mapping of nanobody binding sites on human LRRK2 by CX-MS
Description	As part of an in-depth characterization of nanobodies directed against the human Leucine-rich repeat kinase 2 (LRRK2), epitopes of the nanobodies bound to LRRK2 were mapped by chemical crosslinking combined with mass spectrometry using the CID-cleavable crosslinker disuccinimidyl sulfoxide (DSSO).
HostingRepository	PRIDE
AnnounceDate	2022-03-10
AnnouncementXML	Submission_2022-03-10_09:08:51.615.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Johannes Gloeckner
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-30 08:32:32	ID requested
⏵ 1	2022-03-10 09:08:52	announced

Singh RK, Soliman A, Guaitoli G, St, ö, rmer E, von Zweydorf F, Dal Maso T, Oun A, Van Rillaer L, Schmidt SH, Chatterjee D, David JA, Pardon E, Schwartz TU, Knapp S, Kennedy EJ, Steyaert J, Herberg FW, Kortholt A, Gloeckner CJ, Vers, é, es W, Nanobodies as allosteric modulators of Parkinson's disease-associated LRRK2. Proc Natl Acad Sci U S A, 119(9):(2022) [pubmed]

submitter keyword: LRRK2, nanobodies, DSSO, CX-MS, crosslinking, CID-cleavable, CL-MS

Christian Johannes Gloeckner
contact affiliation	German Center for Neurodegenerative Diseases (DZNE) Otfried-Müller-Strasse 23 72076 Tübingen Germany
contact email	johannes.gloeckner@dzne.de
lab head
Johannes Gloeckner
contact affiliation	DZNE
contact email	johannes.gloeckner@dzne.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD030063

PRIDE project URI

• PRIDE
  1. PXD030063
     1. Label: PRIDE project
     2. Name: Mapping of nanobody binding sites on human LRRK2 by CX-MS