PXD030053

PXD030053 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Deciphering the Physiological Response of Escherichia coli Under High ATP Demand
Description	One longstanding question in microbiology is how microbes buffer perturbations in energy metabolism. In this study, we systematically analyzed the impact of different levels of ATP demand in Escherichia coli under various conditions (aerobic and anaerobic, with and without cell growth). One key finding is that, under all conditions tested, the glucose uptake increases with rising ATP demand, but only to a critical level beyond which it drops markedly, even below wild-type levels. Focusing on anaerobic growth and using metabolomics and proteomics data in combination with a kinetic model, we show that this biphasic behavior is induced by the dual dependency of the phosphofructokinase on ATP (substrate) and ADP (allosteric activator). This mechanism buffers increased ATP demands by a higher glycolytic flux but, as shown herein, it collapses under very low ATP concentrations. Model analysis also revealed two major rate-controlling steps in the glycolysis under high ATP demand, which could be confirmed experimentally. Our results provide new insights on fundamental mechanisms of bacterial energy metabolism and guide the rational engineering of highly productive cell factories.
HostingRepository	MassIVE
AnnounceDate	2021-11-30
AnnouncementXML	Submission_2021-11-30_02:28:53.369.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Witold Szymanski
SpeciesList	scientific name: Escherichia coli MG1665;
ModificationList	unknown modification
Instrument	Q Exactive Plus

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-30 01:35:14	ID requested
⏵ 1	2021-11-30 02:28:53	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Escherichia coli, LC-MSMS, stress, drug, QExactive Plus

submitter keyword: Escherichia coli, LC-MSMS, stress, drug, QExactive Plus

Contact List

Steffen Klamt
contact affiliation	MPI Magdeburg
contact email	klamt@mpi-magdeburg.mpg.de
lab head
Witold Szymanski
contact affiliation	Max Planck Institute for Terrestrial Microbiology
contact email	witold.szymanski@mpi-marburg.mpg.de
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000088475/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000088475/