PXD030041

PXD030041 is an original dataset announced via ProteomeXchange.

Title	BCAS0292 is involved in the adaptation of Burkholderia cenocepacia and shows characteristics of a DNA mimic protein AFFINITY PURIFICATION
Description	Adaptation of opportunistic pathogens to their host environment requires reprogramming of a vast array of genes to facilitate host survival in the host. Burkholderia cenocepacia, a Gram-negative bacterium with a large genome of ~ 8Mb in size that colonizes environmental niches, is exquisitely adaptable to the hypoxic environment of the cystic fibrosis lung and survives in macrophages. We previously identified an immunoreactive acidic protein encoded on replicon 3, BCAS0292. Deletion of the BCAS0292 gene resulted in altered abundance of >1000 proteins; 46 proteins became undetectable while 556 proteins showed >1.5-fold reduced abundance, suggesting BCAS0292 is a global regulator. Moreover, the ∆BCAS0292 mutant showed a range of pleiotropic effects: virulence and host-cell attachment were reduced, antibiotic susceptibility was altered and biofilm formation enhanced. Its growth and survival were impaired in 6% oxygen. Structural analysis revealed BCAS0292 presents a dimeric β-structure with a negative surface charge. The ΔBCAS0292 mutant displayed altered DNA supercoiling, implicated in global regulation of gene expression. Five proteins were identified in pull-downs with FLAG-tagged BCAS0292, including the Histone H1-like protein, HctB, recognised as a global transcriptional regulator. Taken together, we propose that BCAS0292 which is increased in abundance in response to stress, acts as a DNA-mimic and binds to DNA-binding proteins, altering DNA topology and regulating the expression of multiple genes, thereby enabling the adaptation of B. cenocepacia to highly diverse environments.
HostingRepository	PRIDE
AnnounceDate	2022-05-27
AnnouncementXML	Submission_2022-05-27_10:48:44.824.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD030041
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Eugene Dillon
SpeciesList	scientific name: Burkholderia cenocepacia; NCBI TaxID: 95486;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-29 07:55:09	ID requested
⏵ 1	2022-05-27 10:48:45	announced

Dataset with its publication pending

submitter keyword: Burkholderia, DNA-protein interactions, host-pathogen interactions

respiratory infections

bacterial adaptation;

Siobhán McClean
contact affiliation	Associate Professor, Head of Biochemistry, School of Biomolecular and Biomedical Science, Associate Dean for Widening Participation, College of Science Conway Institute room F059 University College Dublin +353 1 716 6723
contact email	siobhan.mcclean@ucd.ie
lab head
Eugene Dillon
contact affiliation	UCD
contact email	eugene.dillon@ucd.ie
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD030041
     1. Label: PRIDE project
     2. Name: BCAS0292 is involved in the adaptation of Burkholderia cenocepacia and shows characteristics of a DNA mimic protein AFFINITY PURIFICATION