PXD030027

PXD030027 is an original dataset announced via ProteomeXchange.

Title	An antibody-free enrichment approach of monomethyllysine peptides base on reductive glutaraldehydation and hydrazide chemistry
Description	Protein lysine monomethylation is an important post-translational modification participated in regulating many biological processes thus there is growing interest in identifying these methylation events. However, the introduction of one methyl group on lysine residues has negligible effect on changing the physical and chemical properties of proteins or peptides, making enriching and identifying monomethylated lysine (Kme1) proteins or peptides extraordinarily challenging. In this study, we developed an antibody-free chemical proteomics approach to effectively capture Kme1 peptides, involving reductive glutaraldehydation of peptides and hydrazide chemistry. By optimizing the reaction condition of reductive glutaraldehydation, aldehyde modified Kme1 residues and piperidine modified primary amines were generated at the same time so that solid-phase hydrazide chemistry could be applied to effectively capture Kme1 peptides from complex mixture. Using this chemical approach, we can enrich and detect Kme1 peptide from peptide mixture containing 5000-fold bovine serum albumin tryptic digest.
HostingRepository	iProX
AnnounceDate	2021-11-26
AnnouncementXML	Submission_2021-11-28_22:10:52.627.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhouxian Li
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	monomethylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-28 22:10:29	ID requested
⏵ 1	2021-11-28 22:10:53	announced

Dataset with its publication pending

submitter keyword: enrichment of monomethyllysine peptides, reductive glutaraldehydation, hydrazide chemistry

Weibing Zhang
contact affiliation	East China University of Science and Technology
contact email	weibingzhang@ecust.edu.cn
lab head
Zhouxian Li
contact affiliation	East China University of Science and Technology
contact email	lizhouxian17@163.com
dataset submitter

iProX dataset URI