PXD030026

PXD030026 is an original dataset announced via ProteomeXchange.

Title	Multifunctional polyphenol-based silk hydrogel alleviates oxidative stress and enhances endogenous regeneration of osteochondral defects
Description	The native microenvironment following osteochondral defect is not permissive for its endogenous regeneration. A variety of hydrogels have been developed to regulate cell behaviors; however, there is still a lack of hydrogels with multifunctional properties that can provide a supportive microenvironment for endogenous regeneration of osteochondral defects. In this study, a multifunctional polyphenol-based silk hydrogel was developed and its effectiveness on in situ osteochondral regeneration was investigated. Silk fibroin (SF) was crosslinked by tannic acid (TA) to form a hydrogen-bonded supramolecular network, followed by enzymatic crosslinking using horseradish peroxidase (HRP)/H2O2 to form a stable SF-TA hydrogel. In vitro investigations showed that the proposed SF-TA hydrogel exhibited a multitude of biological effects including scavenging reactive oxygen species (ROS), maintaining cell viability, and promoting the proliferation of bone marrow mesenchymal stem cells (BMSCs), as a result of the incorporation of the bioactive TA molecule. Besides, TA had multiple phenolic hydroxyl groups that formed interactions with the therapeutic molecule E7 peptide which improved BMSCs migration when continuously released from the SF-TA hydrogel. In vivo, after implantation for 12-weeks into a rabbit osteochondral defect, the SF-TA hydrogels promoted enhanced regeneration of both cartilage and subchondral bone as compared to hydrogels without TA incorporation. These findings indicated that the multifunctional SF-TA hydrogel provided a microenvironment suitable for the endogenous regeneration of osteochondral defects, thus the SF-TA hydrogel exhibited promising properties for future clinical applications.
HostingRepository	iProX
AnnounceDate	2021-11-28
AnnouncementXML	Submission_2022-07-28_05:24:24.129.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wei Zhang
SpeciesList	scientific name: Rattus rattus; NCBI TaxID: 10117;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-28 22:01:48	ID requested
1	2021-11-28 22:02:15	announced
⏵ 2	2022-07-28 05:24:24	announced	2022-07-28: Update publication information.

Zhang W, Zhang Y, Li X, Cao Z, Mo Q, Sheng R, Ling C, Chi J, Yao Q, Chen J, Wang H, Multifunctional polyphenol-based silk hydrogel alleviates oxidative stress and enhances endogenous regeneration of osteochondral defects. Mater Today Bio, 14():100251(2022) [pubmed]

submitter keyword: Osteochondral regeneration, Hydrogel, Microenvironment, Oxidative stress, Tannic acid, BMSCs

Hongmei Wang
contact affiliation	Southeast University
contact email	wanghongmei85@163.com
lab head
Wei Zhang
contact affiliation	Southeast University
contact email	zhang.wei@seu.edu.cn
dataset submitter

iProX dataset URI